A Method for Isolation and Purification of Peanut Genomic DNA Suitable for Analytical Applications

Sharma, K K and Lavanya, M and Anjaiah, V (2000) A Method for Isolation and Purification of Peanut Genomic DNA Suitable for Analytical Applications. Plant Molecular Biology Reporter, 18 (4). 393a-393h. ISSN 1572-9818

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Abstract

Numerous methods are available for isolation of plant genomic DNA, but in practice these procedures are empirical due to variability in plant tissue composition. Consistent isolation of quality DNA from peanut (Arachis hypogaea L.) is particularly problematic due to the presence of phenolic compounds and polysaccharides. Inconsistencies in extraction results can be attributed to the age and growth stage of the plant material analyzed. Mature leaves have higher quantities of polyphenols, tannins, and polysaccharides that can contaminate DNA during isolation. We show that four published protocols could not be used to isolate peanut DNA of sufficient quality for PCR amplification or Southern hybridization. We have devised a new protocol that uses DEAE-cellulose purification to isolate peanut DNA useful for downstream applications.

Item Type:	Article
Divisions:	UNSPECIFIED
CRP:	UNSPECIFIED
Uncontrolled Keywords:	DNA isolation, PCR amplification, peanut, Southern hybridization, transgenic plants
Subjects:	Mandate crops > Groundnut
Depositing User:	Library ICRISAT
Date Deposited:	26 Sep 2011 04:15
Last Modified:	26 Sep 2011 04:15
URI:	http://oar.icrisat.org/id/eprint/1921
Official URL:	http://dx.doi.org/10.1007/BF02825068
Projects:	UNSPECIFIED
Funders:	OPEC, Asian Development Bank
Acknowledgement:	We thank Dr. Rodomiro Ortiz for his encouragement and suggestions on the manuscript. Part of the funding for this work was provided by grants from OPEC and Asian Development Bank.
Links:	Author

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