Mathur, K and Thakur, R P and Rao, V P (1999) A simple method for long-term preservation of cultures of Colletotrichum graminicola and C. gloeosporioides [Glomeralla cingulata] causing anthracnose in sorghum. International Sorghum and Millets Newsletter, 40. pp. 43-44.
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Abstract
Long-term preservation and maintenance of pathogen isolates is essential for studies on pathogenic variability and virulence monitoring. Once the pathogen isolates are appropriately characterized for their morphologic, pathogenic, and genetic diversity they become reference isolates for future studies. Frequent subculturing of necrotrophic pathogens is not only laborious, but it can also induce spontaneous natural mutations in actively growing cultures. There are numerous reports of reduced viability and pathogenicity of isolates due to repeated subculturing on synthetic media. In our studies on pathogenic variability in Colletotrichum graminicola (Ces.) Wils. and C. gloeosporioides (Penz.) Sacc., the causal agents of anthracnose of sorghum (Mathur et al. 1998), we faced the problem of adequately maintaining a large number of isolates (>200) by repeated subculturing on oatmeal agar (OMA) at monthly intervals.
| Item Type: | Article |
|---|---|
| Divisions: | UNSPECIFIED |
| CRP: | UNSPECIFIED |
| Subjects: | Mandate crops > Sorghum |
| Depositing User: | Library ICRISAT |
| Date Deposited: | 20 Sep 2011 09:20 |
| Last Modified: | 20 Sep 2011 09:20 |
| URI: | http://oar.icrisat.org/id/eprint/1781 |
| Official URL: | |
| Projects: | UNSPECIFIED |
| Funders: | UNSPECIFIED |
| Acknowledgement: | UNSPECIFIED |
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