Characterization of the A4 cytoplasmic male-sterile lines of sorghum using RFLP of mtDNA

Sivaramakrishnan, S and Seetha, K and Reddy, B V S (1997) Characterization of the A4 cytoplasmic male-sterile lines of sorghum using RFLP of mtDNA. Euphytica, 93 (3). pp. 301-305. ISSN 1573-5060

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Abstract

Three sorghum cytoplasmic male sterile lines CSV4 A(V), CSV4 A(G1) and CSV4 A(M), grouped as A4, were compared with amilo (A1) and two other non-milo (A2 andA3) cytoplasms for their RFLP patterns ofmitochondrial DNA (mtDNA). A 9.7 kb clone from pearl millet mtDNA discriminated each of the three A4 entries whereas other maize and pearl millet mtDNA clones used could not distinguish this group completely. The molecular differences within the A4 cytoplasmic group offer some explanation for the inconsistency in the fertility restoration behaviour of these A4 lines obtained with a definite set of testers in the field.

Item Type:	Article
Divisions:	UNSPECIFIED
CRP:	UNSPECIFIED
Uncontrolled Keywords:	Sorghum bicolor, RFLP, cytoplasmic male sterility, sorghum, mitochondrial DNA
Subjects:	Mandate crops > Sorghum
Depositing User:	Mr Sanat Kumar Behera
Date Deposited:	16 Sep 2011 09:25
Last Modified:	16 Sep 2011 09:25
URI:	http://oar.icrisat.org/id/eprint/1641
Official URL:	http://dx.doi.org/10.1023/A:1002906606333
Projects:	UNSPECIFIED
Funders:	UNSPECIFIED
Acknowledgement:	We thank Mr. K.D.V. Prasad for cluster analysis and Mr. R. Luke for technical assistance.
Links:	Author

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