Development and characterization of polymorphic microsatellite markers in taro (Colocasia esculenta)

Mace, E S and Godwin, I D (2002) Development and characterization of polymorphic microsatellite markers in taro (Colocasia esculenta). Genome, 45 (5). pp. 823-832. ISSN 1480-3321

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Abstract

Microsatellite-containing sequences were isolated from enriched genomic libraries of taro (Colocasia esculenta (L.) Schott). The sequencing of 269 clones yielded 77 inserts containing repeat motifs. The majority of these (81.7%) were dinucleotide or trinucleotide repeats. The GT/CA repeat motif was the most common, accounting for 42% of all repeat types. From a total of 43 primer pairs designed, 41 produced markers within the expected size range. Sixteen (39%) were polymorphic when screened against a restricted set of taro genotypes from Southeast Asia and Oceania, with an average of 3.2 alleles detected on each locus. These markers represent a useful resource for taro germplasm management, genome mapping, and marker-assisted selection.

Item Type:	Article
Divisions:	UNSPECIFIED
CRP:	UNSPECIFIED
Subjects:	Others > Genetics and Genomics
Depositing User:	Library ICRISAT
Date Deposited:	27 Sep 2011 05:25
Last Modified:	27 Sep 2011 05:25
URI:	http://oar.icrisat.org/id/eprint/1542
Official URL:	http://dx.doi.org/10.1139/G02-045
Projects:	UNSPECIFIED
Funders:	Australian Centre for International Agricultural Research
Acknowledgement:	The authors gratefully acknowledge the support of the Australian Centre for International Agricultural Research (ACIAR) project CS2/94/43, and the collaboration received through the AusAID-funded Taro Genetic Resources project. Additonally, the technical support provided by Dr. Giovanni Cordeiro and Dr. Tina Maguire is gratefully acknowledged.
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