In-silico Sequence Alignment, Phylogeny, Docking, and Domain Analysis of Fatty Acid Desaturase (FAD) Proteins in Pearl Millet (Pennisetum glaucum L.)

    Vasanthrao, T and Moin, M and Kona, P and Tyagi, W and Gupta, S K and Palakolanu, S R and Danam, S B (2025) In-silico Sequence Alignment, Phylogeny, Docking, and Domain Analysis of Fatty Acid Desaturase (FAD) Proteins in Pearl Millet (Pennisetum glaucum L.). International Journal of Bio-resource and Stress Management, 16 (10). 01-13. ISSN 0976-3988

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    Abstract

    This in-silico analysis was conducted during May and June, 2025 at ICRISAT, Patancheru, Telangana, India to explore the properties of pearl millet FAD (fatty acid desaturase) proteins. The protein sequences were retrieved from the NovoGene Millet database, and analyzed using ExPASy ProtParamto investigate the FAD protein family in pearl millet. Phyre2.1 was used to predict the secondary structures, and protein phylogeny was analyzed using ClustalW to identify evolutionary relationships. Conserved motifs were identified using the MEME online tool. Multiple sequence alignment of PgFAD protein sequences across six genotypes in the pangenome was performed through ClustalW to identify the polymorphism. A total of 22 PgFAD proteins were identified, and their physiochemical analysis revealed diverse molecular weights, isoelectric points (pI) and hydrophobicity profiles, suggested functional variability. Three-dimensional secondary structure analysis revealed that all PgFADs are rich in alpha helical content (45–66%), suggesting a compact, stable and membrane spanning structure. Low to moderate disordered regions (6 to 22%) in these proteins indicated their enzymatic activities. Protein polymorphism analysis showed that PgFAD-5e and PgFAD-6c are conserved across all six genotypes in the pangenome, whereas PgFAD-5c, PgFAD-5f and PgFAD-1w6 exhibited multiple nonsynonymous mutations particularly in PI526529 genotype. These findings provided insights into the structural and functional diversity of fatty acid desaturases and established a foundation for targeting these genes to enhance lipid stability and improve quality-related traits of pearl millet.

    Item Type: Article
    Divisions: Global Research Program - Accelerated Crop Improvement
    CRP: UNSPECIFIED
    Uncontrolled Keywords: FADs, Pennisetum glaucum, lipid metabolism, phylogeny
    Subjects: Others > Genetic Engineering
    Mandate crops > Millets > Pearl Millet
    Others > Genetics and Genomics
    Depositing User: Mr Nagaraju T
    Date Deposited: 10 Aug 2026 05:54
    Last Modified: 10 Aug 2026 05:54
    URI: http://oar.icrisat.org/id/eprint/13657
    Official URL: https://indianjournals.com/article/ijbsm-16-10-028
    Projects: UNSPECIFIED
    Funders: UNSPECIFIED
    Acknowledgement: Thalari Vasanthrao acknowledges the UGC-CSIR, Govt. of India for providing fellowship. The authors also express their gratitude to the PJTAU, Hyderabad, ICRISAT, Patancheru, Hyderabad. All the authors have read and approved the manuscript.
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