Kummari, D and Kaliamoorthy, S and Pasha, G and Dhaware, M G and Katamreddy, S C and Kavi Kishor, P B and Sharma, K K and Vadez, V and Reddy, P S (2026) Functional characterization of sorghum aquaporin SbTIP2;1 for enhanced salt, drought and heat tolerance in transgenic tobacco. Plant Science, 364. ISSN 1873-2259
Full text not available from this repository. (Request a copy)Abstract
Aquaporins (AQPs) significantly impact the regulation of water transport and can modulate traits related to tolerance to abiotic stress. Our previous study confirmed that SbTIP2;1 is expressed in response to abiotic stress conditions in sorghum. This study examined the role of the SbTIP2;1 gene in transgenic tobacco plants. The SbTIP2;1 gene has been cloned into the Gateway-compatible plant expression vector pMDC100, driven by the CaMV35S promoter, and subsequently transferred into tobacco plants using the leaf-disc method. Tobacco plants expressing the SbTIP2;1 gene were propagated to the T2 generation and exposed to increasing drought, salinity, heat, and cold stresses. Water use traits, including transpiration, canopy temperature (CT), canopy temperature difference (CTD), and expression profiles, showed significant differences among transgenic tobacco events and wild-type (WT) plants under abiotic stress treatments. Overexpression of the SbTIP2;1 gene in roots and leaves showed that transgenic plants regulate transpiration differently than WT under drought and heat stress, enabling more water conservation and/or better leaf cooling. Transgenics showed cooler canopy temperature (CT) and higher canopy temperature depression (CTD) compared to WT, along with significant upregulation of SbTIP2;1 under stress, underscoring its role in improving adaptation to drought and heat. Therefore, the sorghum AQP gene SbTIP2;1 may serve as a promising candidate for enhancing abiotic stress tolerance in crops.
| Item Type: | Article |
|---|---|
| Divisions: | Global Research Program - Accelerated Crop Improvement |
| CRP: | UNSPECIFIED |
| Uncontrolled Keywords: | sorghum aquaporin, drought tolerance, heat tolerance |
| Subjects: | Others > Abiotic Stress Mandate crops > Sorghum |
| Depositing User: | Mr Nagaraju T |
| Date Deposited: | 18 May 2026 04:13 |
| Last Modified: | 18 May 2026 04:13 |
| URI: | http://oar.icrisat.org/id/eprint/13625 |
| Official URL: | https://www.sciencedirect.com/science/article/abs/... |
| Projects: | UNSPECIFIED |
| Funders: | UNSPECIFIED |
| Acknowledgement: | PR acknowledges financial support from the Department of Science and Technology, Government of India, for a fellowship and research grant through the INSPIRE Faculty Award No. IFALSPA-06, Young Scientist Award SB/YS/LS-12/2013, and partial support from the core grant research (CRG) grant file No. DST No: EMR/20l6/006726. KS was supported by the Department of Science and Technology, Science and Engineering Research Board (DST-SERB), specifically through the National Postdoctoral Fellowship... |
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