Wasudeo, N G and Rayaprolu, L and Usha Kiranmayee, K N S and Mehtre, S P and Kalpande, H and Deshpande, S P and Wani, S H (2026) Genetic mapping of fertility restoration (Rf) genes and development-validation of linked SNPs for A1 cytoplasm of sorghum (Sorghum bicolor (L.) Moench). Journal of Applied Genetics. pp. 1-14. ISSN 2190-3883
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Abstract
Sorghum (Sorghum bicolor L. Moench) is one of the most important cereal crops in which cytoplasmic genetic male sterility (CGMS) is widely exploited for hybrid breeding. Commercial sorghum hybrids globally are predominantly based on the A1 cytoplasmic genetic male sterility (CGMS) system because of its operational ease and cytoplasmic stability. To map fertility restoration (Rf) loci, hybrids were developed by crossing the CMS line 296 A with a recombinant inbred line (RIL) population derived from the cross 296B × IS18551, which segregates for fertility restoration genes. The RIL population consisted of advanced-generation lines (F₆:₇), ensuring high homozygosity suitable for genetic mapping. In addition, F₂ and BC₁F₁ populations were developed from selected fertile RIL-derived hybrids to validate fertility restoration loci. The RIL population was genotyped using a genotyping-by-sequencing (GBS) approach and employed for genome-wide genetic mapping of fertility restoration genes. Based on significant associations, a set of 19 single nucleotide polymorphism (SNP) markers associated with fertility restoration was selected and converted into Kompetitive Allele-Specific PCR (KASP) assays. These 19 KASP markers were first validated in the RIL population and subsequently evaluated using a diverse panel of 150 sorghum lines, comprising 72 maintainer (B) lines and 78 restorer (R) lines. Of the 19 validated KASP markers, six markers were associated with the major fertility restoration locus Rf1 on chromosome SBI-08, while the remaining markers were linked to loci located on chromosomes SBI-01, SBI-03, SBI-07, SBI-09, and SBI-10. The results clearly demonstrate the ability of specific SNP markers to discriminate fertility restoration responses between B- and R-lines. The identified polymorphic SNP markers provide robust molecular tools for fast-tracking line conversion and marker-assisted selection in sorghum hybrid breeding programs. Overall, this study enhances the current understanding of fertility restoration genetics in sorghum by identifying key Rf loci, improving hybrid breeding efficiency, and providing new insights into cytoplasm–nuclear interactions governing male fertility restoration.
| Item Type: | Article |
|---|---|
| Divisions: | Global Research Program - Accelerated Crop Improvement |
| CRP: | UNSPECIFIED |
| Uncontrolled Keywords: | CGMS, SNP, KASP, GWAS, Rf |
| Subjects: | Others > Plant Breeding Mandate crops > Sorghum Others > Genetics and Genomics |
| Depositing User: | Mr Nagaraju T |
| Date Deposited: | 29 Apr 2026 03:59 |
| Last Modified: | 29 Apr 2026 03:59 |
| URI: | http://oar.icrisat.org/id/eprint/13610 |
| Official URL: | https://link.springer.com/article/10.1007/s13353-0... |
| Projects: | UNSPECIFIED |
| Funders: | UNSPECIFIED |
| Acknowledgement: | UNSPECIFIED |
| Links: |
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