Multivariate stability analysis to select elite rice (Oryza sativa L.) genotypes for grain yield, zinc and Iron

Singh, Akansha and Singh, D K and Singh, S K and Singh, V K and Kumar, A (2025) Multivariate stability analysis to select elite rice (Oryza sativa L.) genotypes for grain yield, zinc and Iron. Scientific Reports (TSI), 15. pp. 1-14. ISSN 2045-2322

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Abstract

The present study was conducted to evaluate 30 rice genotypes at three different locations in eastern Uttar Pradesh during the Wet- 2020–21 and determine the impact of GEI on grain yield (tha-1), days to 50% flowering, grain Fe content (PPM), and grain Zn content (PPM). The study also aimed to identify the genotypes that displayed the best performance according to the multi-trait stability index (MTSI), multi-trait genotype-ideotype distance index (MGIDI), and factor analysis and ideotype-design (FAI-BLUP) index. AMMI analysis demonstrated significant variation for environment (E), genotype (G), and genotype-by-environment interaction (GEI) (P < 0.01) for all the studied traits. The AMMI1 biplot showed that PC1 explained the majority of the variation for GY (77.6%), DTF (90.5%), Fe (73.5%), and Zn (86.8%), helping to identify stable and high-performing genotypes. AMMI2 biplot further resolved complex GEI patterns, highlighting genotypes with specific adaptability to individual environments. The GGE biplot revealed clear “which-won-where” patterns for GY, DTF, Fe, and Zn, explaining 94.37%, 99.71%, 83.49%, and 96.93% of GEI variation, respectively. BLUP analysis using a linear mixed model revealed significant GEI effects for GY, DTF, Fe, and Zn across 30 rice genotypes in three environments. Low heritability was observed for Fe (28.2%) and moderate for GY (54.4%) and Zn (56.4%), while DTF showed high heritability with strong genotypic accuracy. Genotype G7 was identified as stable, early, high-yielding, and rich in Fe based on HMGV, RPGV, and HMRPGV indices. The MTSI, MGIDI and FAI-BLUP analysis revealed that BHU-SKS-1 (G15) and IR105696 -1–2-3–1-1–1 -B (G9) were the most stable and best mean performer for high grain yield and high grain Fe & Zn content, while IR 108,195–3-1–1-2 (G7) was the most stable and best mean performer for high grain yield and high grain Fe content with early flowering.

Item Type:	Article
Divisions:	Others
CRP:	UNSPECIFIED
Uncontrolled Keywords:	AMMI, Biofortification, Ideotype indices, Rice, WAASB
Subjects:	Others > Rice Others > Biofortification
Depositing User:	Mr Nagaraju T
Date Deposited:	03 Mar 2026 04:27
Last Modified:	03 Mar 2026 04:27
URI:	http://oar.icrisat.org/id/eprint/13509
Official URL:	https://www.nature.com/articles/s41598-025-11748-7
Projects:	HarvestPlus Project during 2020–2021
Funders:	IFPRI (Washington D.C.) & CIAT (Columbia)
Acknowledgement:	Authors are thankful to IFPRI (Washington D.C.) & CIAT (Columbia) for funding through HarvestPlus Project. AS is thankful to Department of Biotechnology, GOI for giving opportunity to work at BHU, Varanasi.
Links:	Google Scholar

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