Bhadragiri, H B and Veerendrakumar, H V and Muluguri, S and Mwololo, J and Monyo, E and Njoroge, S and Sudini, H K and Bhat, R and Kar, J and Pradhan, S K and Mayes, S and Pandey, M K (2025) Multi-locus Genome-Wide Association Study Uncovers Candidate Genes for Early Leaf Spot and Rust Resistance in Groundnut. In: Advances in Arachis through Genomics and Biotechnology (AAGB-2025), 23-25 March 2025, Novotel, Goa, India.
Full text not available from this repository. (Request a copy)Abstract
Early leaf spot (ELS) and rust, caused by Cercospora arachidicola and Puccinia arachidis respectively, are major fungal diseases limiting global groundnut (Arachis hypogaea L.) production. Mini-core set was evaluated for ELS at 3 locations Senegal, Malawi and Mali and for rust at Dharwad for three seasons using the modified 9-point scale. High-density genotyping was performed using whole genome resequencing data to identify genomic regions associated with early leaf spot and rust resistance. After quality filtering, 561,009 high-confidence SNPs were obtained. To dissect the genetic architecture of resistance to these diseases, genome-wide association study (GWAS) was conducted on 184 diverse groundnut mini-core lines which revealed four marker trait associations (MTAs) significantly associated with ELS resistance and twelve MTAs associated with rust resistance, collectively explaining 10–59% of the phenotypic variance. The most significant SNPs were Ah01_48511619 and Ah17_133493906 for rust resistance, which were clearly differentiating the resistant and susceptible minicore lines and also validated in other diverse populations. These SNPs encode sterol c4-methyl oxidase 1-2 and MYB transcription factor. Sterol c4-methyl oxidase 1-2 is involved in brassinosteroids biosynthesis which inturn has a role in salicylic acid pathway and MYB transcription factor involved in activation of WRKY disease resistance genes to combat against fungal pathogens. For ELS resistance, the most significant SNP (Ah14_140021024) is associated with MYB transcription factor explaining phenotypic variance of 59%. The validated markers and the predicted candidate genes can be useful in integrating favorable alleles into elite cultivars through marker-assisted selection (MAS) for the development of cultivars with improved disease resistance. The ELS markers can be utilized in the breeding programs upon further validation.
| Item Type: | Conference or Workshop Item (Speech) |
|---|---|
| Divisions: | Center of Excellence in Genomics and Systems Biology |
| CRP: | UNSPECIFIED |
| Uncontrolled Keywords: | Minicore, Genome wide association studies, Marker trait associations, Candidate genes, Marker-assisted selection |
| Subjects: | Mandate crops > Groundnut Others > Genetics and Genomics |
| Depositing User: | Mr Nagaraju T |
| Date Deposited: | 04 Jul 2025 09:05 |
| Last Modified: | 04 Jul 2025 09:05 |
| URI: | http://oar.icrisat.org/id/eprint/13204 |
| Acknowledgement: | UNSPECIFIED |
| Links: | |
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