Huai, D and Huang, L and Xue, X and Yu, B and Ding, Y and Jin, G and Liu, H and Pandey, M K and Sudini, H K and Luo, H and Zhou, X and Liu, N and Chen, W and Yan, L and Chen, Y and Wang, X and Wang, Q and Kang, Y and Wang, Z and Chen, X and Jiang, H and Lei, Y and Liao, B (2025) Identification of candidate genes associated with resistance to aflatoxin production in peanut through genetic mapping and transcriptome analysis. Theoretical and Applied Genetics (TSI), 138. pp. 1-13. ISSN 0040-5752
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Abstract
Peanut (Arachis hypogaea L.) is a globally significant oil and economic crop, serving as a primary source of edible oil and protein. Aflatoxin contamination is a main risk factor for peanut food safety and industry development worldwide. The most cost-economic and effective control strategy entails the exploration and utilization of natural resistance in peanut, alongside the development of resistant varieties. However, the underlying mechanism of resistance to aflatoxin production (AP) in peanuts remains elusive. In this study, a RIL population derived from a cross between Zhonghua 10 (susceptible) and ICG 12625 (resistant), was used to identify quantitative trait loci (QTLs) for AP resistance. Overall, seven QTLs associated with AP resistance were mapped on five chromosomes, explaining 6.83–17.86% of phenotypic variance (PVE). Notably, only two major QTLs, namely qAftA07and qAftB06.2, were consistently detected across different environments with 6.83–16.52% PVE. To predict the candidate genes for AP resistance in qAftA07and qAftB06.2, the transcriptome analysis of seeds from parental lines inoculated with Aspergillus flavus were conducted. A total of 175 and 238 candidate genes were respectively identified in qAftA07 and qAftB06.2, encompassing genes with non-synonymous genomic variations as well as differentially expressed genes. Combined with the weighted gene co-expression network analysis, 10 and 11 genes in qAftA07 and qAftB06.2 were characterized showing a high correlation with aflatoxin content, thereby representing the most promising candidate genes within these two QTLs. These results provide valuable insights for future map-based cloning studies targeting candidate genes associated with AP resistance in peanut.
| Item Type: | Article |
|---|---|
| Divisions: | Center of Excellence in Genomics and Systems Biology Global Research Program - Accelerated Crop Improvement |
| CRP: | UNSPECIFIED |
| Uncontrolled Keywords: | genetic mapping, transcriptome analysis, aflatoxin, peanut |
| Subjects: | Mandate crops > Groundnut Others > Genetics and Genomics Others > Aflatoxins |
| Depositing User: | Mr Nagaraju T |
| Date Deposited: | 06 Jun 2025 11:00 |
| Last Modified: | 06 Jun 2025 11:00 |
| URI: | http://oar.icrisat.org/id/eprint/13130 |
| Official URL: | https://link.springer.com/article/10.1007/s00122-0... |
| Projects: | UNSPECIFIED |
| Funders: | UNSPECIFIED |
| Acknowledgement: | Funding: This work was supported by the National Natural Science Foundation of China (32001510, 32101708, 31461143022 and 32161143006), the National Peanut Industry Technology System Construction (CARS-13), the Key R&D Program of China (2022YFD1200400 and 2023YFD1202800) and Innovation Program of the Chinese Academy of Agricultural Sciences (2024-2060299-089-031). The funders had no role in experiment design, data analysis, decision to publish, or preparation of the manuscript. |
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