Tamhane, V A and Sant, S S and Jadhav, A R and War, A R and Sharma, H C and Jaleel, A and Kashikar, A S (2021) Label-free quantitative proteomics of Sorghum bicolor reveals the proteins strengthening plant defense against insect pest Chilo partellus. Proteome Science, 19. pp. 1-25. ISSN 1477-5956
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Abstract
Background Spotted stem borer- Chilo partellus - a Lepidopteran insect pest of Sorghum bicolor is responsible for major economic losses. It is an oligophagous pest, which bores through the plant stem, causing ‘deadheart’ and hampering the development of the main cob. We applied a label-free quantitative proteomics approach on three genotypes of S. bicolor with differential resistance/ susceptibility to insect pests, intending to identify the S. bicolor’s systemic protein complement contributing to C. partellus tolerance. Methods The proteomes of S. bicolor with variable resistance to insect pests, ICSV700, IS2205 (resistant) and Swarna (susceptible) were investigated and compared using label-free quantitative proteomics to identify putative leaf proteins contributing to resistance to C. partellus. Results The multivariate analysis on a total of 967 proteins led to the identification of proteins correlating with insect resistance/susceptibility of S. bicolor. Upon C. partellus infestation S. bicolor responded by suppression of protein and amino acid biosynthesis, and induction of proteins involved in maintaining photosynthesis and responding to stresses. The gene ontology analysis revealed that C. partellus-responsive proteins in resistant S. bicolor genotypes were mainly involved in stress and defense, small molecule biosynthesis, amino acid metabolism, catalytic and translation regulation activities. At steady-state, the resistant S. bicolor genotypes displayed at least two-fold higher numbers of unique proteins than the susceptible genotype Swarna, mostly involved in catalytic activities. Gene expression analysis of selected candidates was performed on S. bicolor by artificial induction to mimic C. partellus infestation. Conclusion The collection of identified proteins differentially expressed in resistant S. bicolor, are interesting candidates for further elucidation of their role in defense against insect pests.
| Item Type: | Article |
|---|---|
| Divisions: | Research Program : Asia |
| CRP: | UNSPECIFIED |
| Uncontrolled Keywords: | Chilo partellus, Insect pests, in-solution proteomics, Plant defense, Label-free quantitative proteomics |
| Subjects: | Mandate crops > Sorghum Others > Entomology |
| Depositing User: | Mr Nagaraju T |
| Date Deposited: | 05 Jun 2025 04:58 |
| Last Modified: | 05 Jun 2025 04:58 |
| URI: | http://oar.icrisat.org/id/eprint/13123 |
| Official URL: | https://link.springer.com/article/10.1186/s12953-0... |
| Projects: | UNSPECIFIED |
| Funders: | Government of India - Department of Science and Technology |
| Acknowledgement: | This work was supported by the Young Scientist research grant to VT from the Science and Engineering Research Board, Department of Science and Technology, Government of India, India. Grant Number SB/YS/LS-132/2013. The authors express thanks to Dr. Vandana Mhaske for her valuable suggestions and editing of the manuscript. The funding support from the Departmental Research and Development Program (DRDP), Institute of Bioinformatics and Biotechnology, Savitribai Phule Pune University, Pune is acknowledged. |
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