Zhang, S and Ghatak, A and Bazargani, M M and Bajaj, P and Varshney, R K and Chaturvedi, P and Jiang, D and Weckwerth, W (2021) Spatial distribution of proteins and metabolites in developing wheat grain and their differential regulatory response during the grain filling process. The Plant Journal (TSI), 107 (3). pp. 669-687. ISSN 0960-7412
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Abstract
Grain filling and grain development are essential biological processes in the plant’s life cycle, eventually contributing to the final seed yield and quality in all cereal crops. Studies of how the different wheat (Triticum aestivum L.) grain components contribute to the overall development of the seed are very scarce. We performed a proteomics and metabolomics analysis in four different developing components of the wheat grain (seed coat, embryo, endosperm, and cavity fluid) to characterize molecular processes during early and late grain development. In-gel shotgun proteomics analysis at 12, 15, 20, and 26 days after anthesis (DAA) revealed 15 484 identified and quantified proteins, out of which 410 differentially expressed proteins were identified in the seed coat, 815 in the embryo, 372 in the endosperm, and 492 in the cavity fluid. The abundance of selected protein candidates revealed spatially and temporally resolved protein functions associated with development and grain filling. Multiple wheat protein isoforms involved in starch synthesis such as sucrose synthases, starch phosphorylase, granule-bound and soluble starch synthase, pyruvate phosphate dikinase, 14-3-3 proteins as well as sugar precursors undergo a major tissue-dependent change in abundance during wheat grain development suggesting an intimate interplay of starch biosynthesis control. Different isoforms of the protein disulfide isomerase family as well as glutamine levels, both involved in the glutenin macropolymer pattern, showed distinct spatial and temporal abundance, revealing their specific role as indicators of wheat gluten quality. Proteins binned into the functional category of cell growth/division and protein synthesis/degradation were more abundant in the early stages (12 and 15 DAA). At the metabolome level all tissues and especially the cavity fluid showed highly distinct metabolite profiles. The tissue-specific data are integrated with biochemical networks to generate a comprehensive map of molecular processes during grain filling and developmental processes.
| Item Type: | Article |
|---|---|
| Divisions: | Center of Excellence in Genomics and Systems Biology |
| CRP: | UNSPECIFIED |
| Uncontrolled Keywords: | wheat, Triticum aestivum, proteomics, metabolomics, grain filling, seed development, seed coat, embryo, endosperm, cavity fluid |
| Subjects: | Others > Wheat Others > Seeds/Seed Bank |
| Depositing User: | Mr Nagaraju T |
| Date Deposited: | 05 Jun 2025 03:32 |
| Last Modified: | 05 Jun 2025 03:32 |
| URI: | http://oar.icrisat.org/id/eprint/13120 |
| Official URL: | https://onlinelibrary.wiley.com/doi/full/10.1111/t... |
| Projects: | UNSPECIFIED |
| Funders: | UNSPECIFIED |
| Acknowledgement: | Authors are thankful to the gardeners Andreas Schröfl and Thomas Joch for excellent plant cultivation in the glasshouse facility of the Molecular Systems Biology Lab (MOSYS), Department of Functional and Evolutionary Ecology, University of Vienna, Vienna, Austria. SZ is supported by a PhD scholarship provided by the China Scholarship Council (CSC) (grant number: 201706850037) and a completion grant of the Vienna Doctoral School Ecology and Evolution of the Faculty of Life Sciences, University of Vienna, Austria. AG is supported by the European Union Horizon 2020 research and innovation program (ADAPT) under grant agreement number GA 2020 862-858. |
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