Identification of stable resistant donors against necrosis inducing strain of bean common mosaic virus in common bean (Phaseolus vulgaris L.)

Manjunatha, L and Basavaraja, T and Manjunatha, N and Srinivasa, N and Kumar, Vijay and Chandora, R and Singh, M and Singh, N P (2021) Identification of stable resistant donors against necrosis inducing strain of bean common mosaic virus in common bean (Phaseolus vulgaris L.). Archives of Phytopathology and Plant Protection, 54 (15-16). pp. 1033-1046. ISSN 0323-5408

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Abstract

Most of the commercially approved cultivars of Phaseolus vulgaris were susceptible to bean common mosaic virus (BCMV) which results in severe reduction in yield. In the present study, a total of 350 common bean genotypes were screened under natural epiphytotic conditions and artificial inoculation during 2016–2017, 2017–2018 and 2018–2019. Only eleven genotypes viz., EC400445, EC400414, ET4515B, EC540173, IC360831, BD9116291, EC31084, EC541703, ET8409, IC340947 and IC356024 were found stable resistant. The BCMV infection was confirmed through reverse-transcription polymerase chain reaction using coat protein gene amplification. The amplified region was sequenced and analyzed. Percent identity of BCMV-K with reported BCMV strains from different parts of world and based on nucleotide sequence ranged between 94% and 99.70% and maximum identity (99.70%) is observed with the strains of Uttar Pradesh (MT265069) and Himachal Pradesh (FJ387162, EU492546). The identified resistant sources from this study could be utilized as donors to develop BCMV resistant common bean cultivars.

Item Type: Article
Divisions: Center of Excellence in Genomics and Systems Biology
CRP: UNSPECIFIED
Uncontrolled Keywords: Bean common mosaic virus, coat protein, common bean, resistance, donor
Subjects: Others > Plant Virology
Depositing User: Mr Nagaraju T
Date Deposited: 04 Jun 2025 07:45
Last Modified: 04 Jun 2025 07:45
URI: http://oar.icrisat.org/id/eprint/13115
Official URL: https://www.tandfonline.com/doi/abs/10.1080/032354...
Projects: UNSPECIFIED
Funders: Indian Council of Agricultural Research
Acknowledgement: ICAR-National Bureau Plant Genetic Resource, New Delhi for providing germplasm material for this study.
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