Katamreddy, S C and Jose, J and Reddy, B P and Sivasakthi, K and Sanivarapu, H and Dube, N and Gaddameedi, A and Kodukula, V S V P and Yogendra, K and Govindaraj, M and Kholova, J and Choudhury, S R and Kishor, P B K and Varijakshapanicker, P and Bhatnagar-Mathur, P and Kumar, A A and Reddy, P S (2025) Drought-Induced Cyanogenesis in Sorghum (Sorghum bicolor L.): Genotypic Variation in Dhurrin Biosynthesis and Stress Response. Plant, Cell & Environment (TSI). pp. 1-21. ISSN 0140-7791
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Abstract
The accumulation of the livestock-harming cyanogenic glucoside dhurrin in the vegetative tissues limits the use of sorghum as a major pasture crop. This study integrates transcriptomics and metabolomics data from the ICSV 93046, CSH 24-MF and ICSR 14001 genotypes, which differ in drought tolerance and cyanide potential (HCNp), to understand the molecular processes of cyanogenesis under drought stress conditions. While ICSV 93046 showed drought adaptation and reduced HCNp, ICSR 14001 and CSH 24-MF exhibited decreased drought stress tolerance with HCN accumulation. The differentially expressed gene (DEG) data showed drought-related genes were significantly upregulated in ICSV 93046 but downregulated in ICSR 14001. KEGG pathway analysis revealed enriched dhurrin biosynthesis and cyanoamino acid metabolism genes, with higher expression in ICSR 14001 than in ICSV 93046. WGCNA analysis revealed that hub genes are involved in drought-induced signalling components, such as phospholipases (PLPs) and lipoxygenases (LOXs), which are implicated in membrane protection. In drought-sensitive genotypes, stress-induced membrane damages lead to the release of dhurrin into the cytoplasm, thus elevating HCN content and activating defence responses. Conversely, the drought-adapted genotype could mitigate HCN production by averting membrane injury, thereby effectively modulating the oxidative stress and preventing the release of dhurrin into the cytoplasm.
| Item Type: | Article |
|---|---|
| Divisions: | Global Research Program - Accelerated Crop Improvement |
| CRP: | CGIAR Research Program on Grain Legumes and Dryland Cereals (GLDC) |
| Uncontrolled Keywords: | dhurrin biosynthesis, drought stress, HCN potential, embrane damage, metabolomics, transcriptomics, WGCNA |
| Subjects: | Mandate crops > Sorghum Others > Drought |
| Depositing User: | Mr Nagaraju T |
| Date Deposited: | 14 Apr 2025 11:09 |
| Last Modified: | 14 Apr 2025 11:09 |
| URI: | http://oar.icrisat.org/id/eprint/13031 |
| Official URL: | https://onlinelibrary.wiley.com/doi/full/10.1111/p... |
| Projects: | UNSPECIFIED |
| Funders: | UNSPECIFIED |
| Acknowledgement: | We thank ICRISAT, the sorghum breeding unit for the sorghum breeding lines, and the International Livestock Research Institute (ILRI) for their instrumental facility support. We would also like to thank Mallayee Srikanth and Bramhananda Reddy from ICRISAT for their help in conducting the glasshouse experiments. J.J. acknowledges the Council of Scientific and Industrial Research (CSIR), Government of India, for the PhD fellowship. P.S. acknowledges the financial support from the Department of Science and Technology, New Delhi, Government of India, for the support from the core grant research (CRG) grant file no. DST No: CRG/2019/004305. The contributions from J.K. are acknowledged and supported by the internal grant agency of the Faculty of Economics and Management, Czech University of Life Sciences, Prague, Czech Republic, grant number 2023B0005. This study was undertaken as part of the CGIAR Research Program on Grain Legumes and Dryland Cereals (CRP-GLDC), partially supported by CGIAR Fund Donors. |
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