Yu, P and Shinde, H and Dudhate, A and Kamiya, T and Gupta, S K and Liu, S and Takano, T and Tsugama, D (2024) A pearl millet plasma membrane protein, PgPM19, facilitates seed germination through the negative regulation of abscisic acid-associated genes under salinity stress in Arabidopsis thaliana. Planta (TSI), 260. ISSN 0032-0935
Full text not available from this repository. (Request a copy)Abstract
Main conclusion The pearl millet gene PgPM19 inhibits seed dormancy by negatively regulating the ABA biosynthesis and ABA signaling pathways in response to salinity stress in Arabidopsis. Abstract Abscisic acid (ABA) plays a pivotal role in orchestrating plant stress responses and development. However, how the ABA signal is transmitted in response to stresses remains primarily uncertain, particularly in monocotyledonous plants. In this study, PgPM19, a gene whose expression is induced by drought, salinity, heat, and ABA in both leaf and root tissues, was isolated from pearl millet. The expression of PgPM19 in yeast cells did not influence their growth when subjected to mannitol, sorbitol, or NaCl stress. However, Arabidopsis plants overexpressing PgPM19 (PgPM19_OE plants) exhibited increased germination rates, greater fresh weights and longer roots under salinity stress during germination, compared to wild-type (WT) plants. Conversely, the pm19L1 (SALK_075435) mutant, featuring a transfer DNA insertion in a closely related PgPM19 homolog (AT1G04560) in Arabidopsis, demonstrated reduced germination rates and smaller fresh weights under salinity-stressed condition than did WT and PgPM19_OE plants. A pivotal ABA biosynthesis gene, NCED3, ABA signaling pathway genes, such as PYL6 and SnRK2.7, alongside downstream ABI genes and stress-responsive genes RAB28 and RD29, were downregulated in PgPM19_OE plants, as evidenced by both transcriptome analysis and quantitative reverse transcription-PCR. These findings raise the possibility that PgPM19 is involved in regulating seed germination by mediating ABA biosynthesis and signaling pathway in response to salinity stress in Arabidopsis. This study contributes to a better understanding of PgPM19 in response to salinity stress and establishes a foundation for unraveling the crosstalk of stress responses and ABA in Arabidopsis and other plant species.
| Item Type: | Article |
|---|---|
| Divisions: | Global Research Program - Accelerated Crop Improvement |
| CRP: | UNSPECIFIED |
| Uncontrolled Keywords: | Abscisic acid, Abiotic stress, AWPM 19, Pennisetum glaucum, Seed dormancy |
| Subjects: | Mandate crops > Millets > Pearl Millet Others > Genetics and Genomics |
| Depositing User: | Mr Nagaraju T |
| Date Deposited: | 19 Dec 2024 06:49 |
| Last Modified: | 19 Dec 2024 06:49 |
| URI: | http://oar.icrisat.org/id/eprint/12885 |
| Official URL: | https://link.springer.com/article/10.1007/s00425-0... |
| Projects: | UNSPECIFIED |
| Funders: | Japan Society for the Promotion of Sciences |
| Acknowledgement: | We are grateful to the International Crop Research Institute of Semi-Arid Tropics (ICRISAT), India, for providing the pearl millet seeds for this study. Pei Yu also thanks financial support by the China Scholarship Council (CSC). |
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