Comparative Metabolomics to Unravel the Biochemical Mechanism Associated with Rancidity in Pearl Millet (Pennisetum glaucum L.)

Yogendra, K and Sanivarapu, H and Avuthu, T and Gupta, S K and Durgalla, P and Banerjee, R and Raman, A and Tyagi, W (2024) Comparative Metabolomics to Unravel the Biochemical Mechanism Associated with Rancidity in Pearl Millet (Pennisetum glaucum L.). International Journal of Molecular Sciences (TSI), 25. pp. 1-16. ISSN 1661-6596

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Abstract

Despite being a highly nutritious and resilient cereal, pearl millet is not popular among consumers and food industries due to the short shelf-life of flour attributed to rapid rancidity development. The biochemical mechanism underlying rancidity, a complex and quantitative trait, needs to be better understood. The present study aims to elucidate the differential accumulation of metabolites in pearl millet that impact the rancidity process. Metabolite profiling was conducted on ten pearl millet genotypes with varying levels of rancidity—comprising high, low, and medium rancid genotypes—utilizing liquid chromatography and high-resolution mass spectrometry (LC-HRMS) at different accelerated ageing conditions. Through non-targeted metabolomic analysis, crucial metabolites associated with rancidity were identified across various biochemical pathways, including fatty acids, glycerophospholipids, sphingolipids, glycerol lipids, flavonoids, alkaloids, and terpenoids. Notably, metabolites such as fatty aldehydes, fatty alcohols, fatty esters, fatty acyls, fatty esters, and fatty amides were significantly elevated in high rancid genotypes, indicating their involvement in the rancidity process. These fatty acids-related metabolites further break down into saturated and unsaturated fatty acids. Four key fatty acids—stearic, palmitic, linoleic and linolenic acid—were quantified in the ten pearl millet genotypes, confirming their role in rancidity development. This investigation promises novel insights into utilizing metabolomics to understand the biochemical processes and facilitate precision breeding for developing low-rancidity pearl millet lines.

Item Type:	Article
Divisions:	Global Research Program - Accelerated Crop Improvement Global Research Program - Enabling Systems Transformation Statistics, Bio-Informatics & Data Management
CRP:	UNSPECIFIED
Uncontrolled Keywords:	biochemical resistance, climate resilient, fatty acids, flavonoids, metabolomics, rancidity
Subjects:	Mandate crops > Millets > Pearl Millet Others > Climate Resilient Technologies
Depositing User:	Mr Nagaraju T
Date Deposited:	05 Nov 2024 04:24
Last Modified:	05 Nov 2024 04:24
URI:	http://oar.icrisat.org/id/eprint/12851
Official URL:	https://www.mdpi.com/1422-0067/25/21/11583
Projects:	UNSPECIFIED
Funders:	Novo Nordisk Foundation, Indian Council of Agricultural Research (ICAR)
Acknowledgement:	We express our gratitude to the late Saikat Datta-Mazumdar, former Principal Scientist I—Nutrition, Dietary Behavior, and Smart Food at ICRISAT, for fruitful discussions.
Links:	Google Scholar

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