Transcriptome analysis reveals key genes associated with root‑lesion nematode Pratylenchus thornei resistance in chickpea

Channale, S and Kalavikatte, D and Thompson, J P and Kudapa, H and Bajaj, P and Varshney, R K and Zwart, R S and Thudi, M (2021) Transcriptome analysis reveals key genes associated with root‑lesion nematode Pratylenchus thornei resistance in chickpea. Scientific Reports (TSI), 11. pp. 1-11. ISSN 2045-2322

PDF - Published Version Available under License ["licenses_description_cc_attribution" not defined]. Download (7MB)

Abstract

The root-lesion nematode, Pratylenchus thornei, is one of the major plant-parasitic nematode species causing significant yield losses in chickpea (Cicer arietinum). In order to identify the underlying mechanisms of resistance to P. thornei, the transcriptomes of control and inoculated roots of three chickpea genotypes viz. D05253 > F3TMWR2AB001 (resistant advanced breeding line), PBA HatTrick (moderately resistant cultivar), and Kyabra (susceptible cultivar) were studied at 20 and 50 days post inoculation using the RNA-seq approach. On analyzing the 633.3 million reads generated, 962 differentially expressed genes (DEGs) were identified. Comparative analysis revealed that the majority of DEGs upregulated in the resistant genotype were downregulated in the moderately resistant and susceptible genotypes. Transcription factor families WRKY and bZIP were uniquely expressed in the resistant genotype. The genes Cysteine-rich receptor-like protein kinase 10, Protein lifeguardlike, Protein detoxification, Bidirectional sugar transporter Sugars Will Eventually be Exported Transporters1 (SWEET1), and Subtilisin-like protease were found to play cross-functional roles in the resistant chickpea genotype against P. thornei. The identified candidate genes for resistance to P. thornei in chickpea can be explored further to develop markers and accelerate the introgression of P. thornei resistance into elite chickpea cultivars.

Item Type:	Article
Divisions:	Center of Excellence in Genomics and Systems Biology
CRP:	UNSPECIFIED
Uncontrolled Keywords:	Agricultural genetics, Biotechnology, Plant biotechnology, Plant sciences, Plant stress responses
Subjects:	Others > Biotechnology Mandate crops > Chickpea
Depositing User:	Mr Nagaraju T
Date Deposited:	10 Jul 2024 10:11
Last Modified:	10 Jul 2024 10:11
URI:	http://oar.icrisat.org/id/eprint/12764
Official URL:	https://www.nature.com/articles/s41598-021-96906-3
Projects:	UNSPECIFIED
Funders:	Science Engineering Research Board (SERB)
Acknowledgement:	We acknowledge the Grains Research and Development Corporation (GRDC), New South Wales (NSW) Department of Primary Industries (DPI) and Queensland Department of Agriculture and Fisheries, Australia, as the co-owners of the chickpea breeding line D05253>F3TMWR2AB001. We acknowledge the breeding by Mr. Ted Knights, NSW DPI and thank Dr. Kristy Hobson for providing seed for this study. We thank Dr. Kevin Moore, NSW DPI for providing seed of PBA HatTrick and Kyabra. We would like to thank Vinay Kumar at The International Crops Research Institute for the Semi-Arid Tropics (ICRISAT) for library construction and sequencing of RNA samples. SC was supported by a postgraduate research scholarship from University of Southern Queensland and a student award from the Crawford Fund.
Links:

View Statistics

Actions (login required)

View Item