High-throughput diagnostic markers for foliar fungal disease resistance and high oleic acid content in groundnut

Pandey, M K and Gangurde, S S and Shasidhar, Y and Sharma, V and Kale, S M and Khan, A W and Shah, P and Joshi, P and Bhat, R S and Janila, P and Bera, S K and Varshney, R K (2024) High-throughput diagnostic markers for foliar fungal disease resistance and high oleic acid content in groundnut. BMC Plant Biology (TSI), 24. pp. 1-16. ISSN 1471-2229

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Abstract

Background Foliar diseases namely late leaf spot (LLS) and leaf rust (LR) reduce yield and deteriorate fodder quality in groundnut. Also the high oleic acid content has emerged as one of the most important traits for industries and consumers due to its increased shelf life and health benefits. Results Genetic mapping combined with pooled sequencing approaches identified candidate resistance genes (LLSR1 and LLSR2 for LLS and LR1 for LR) for both foliar fungal diseases. The LLS-A02 locus housed LLSR1 gene for LLS resistance, while, LLS-A03 housed LLSR2 and LR1 genes for LLS and LR resistance, respectively. A total of 49 KASPs markers were developed from the genomic regions of important disease resistance genes, such as NBS-LRR, purple acid phosphatase, pentatricopeptide repeat-containing protein, and serine/threonine-protein phosphatase. Among the 49 KASP markers, 41 KASPs were validated successfully on a validation panel of contrasting germplasm and breeding lines. Of the 41 validated KASPs, 39 KASPs were designed for rust and LLS resistance, while two KASPs were developed using fatty acid desaturase (FAD) genes to control high oleic acid levels. These validated KASP markers have been extensively used by various groundnut breeding programs across the world which led to development of thousands of advanced breeding lines and few of them also released for commercial cultivation. Conclusion In this study, high-throughput and cost-effective KASP assays were developed, validated and successfully deployed to improve the resistance against foliar fungal diseases and oleic acid in groundnut. So far deployment of allele-specific and KASP diagnostic markers facilitated development and release of two rust- and LLS-resistant varieties and five high-oleic acid groundnut varieties in India. These validated markers provide opportunities for routine deployment in groundnut breeding programs.

Item Type:	Article
Divisions:	Center of Excellence in Genomics and Systems Biology
CRP:	UNSPECIFIED
Uncontrolled Keywords:	Late leaf spot, Leaf rust, Gene-based markers, Candidate gene discovery, Diagnostic markers
Subjects:	Mandate crops > Groundnut Others > Plant Disease
Depositing User:	Mr Nagaraju T
Date Deposited:	15 Apr 2024 11:17
Last Modified:	15 Apr 2024 11:17
URI:	http://oar.icrisat.org/id/eprint/12624
Official URL:	https://link.springer.com/article/10.1186/s12870-0...
Projects:	UNSPECIFIED
Funders:	UNSPECIFIED
Acknowledgement:	We would like to acknowledge MARS-Wrigley, USA; the Government of Karnataka (GoK), India; the Bill & Melinda Gates Foundation (BMGF), USA; and the Indian Council of Agricultural Research (ICAR), India, for financial support for this research. We are grateful to Richard Whitehead for editing the language of the manuscript.
Links:	Google Scholar

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