Sayiprathap, B R and Patibanda, A K and Kumari, V P and Jayalalitha, K and Ramappa, H K and Rajeswari, E and Karthiba, L and Saratbabu, K and Sharma, M and Sudini, H K (2022) Salient Findings on Host Range, Resistance Screening, and Molecular Studies on Sterility Mosaic Disease of Pigeonpea Induced by Pigeonpea sterility mosaic viruses (PPSMV-I and PPSMV-II). Frontiers in Microbiology (TSI), 13. pp. 1-13. ISSN 1664-302X
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Abstract
Two distinct emaraviruses, Pigeonpea sterility mosaic virus-I (PPSMV-I) and Pigeonpea sterility mosaic virus-II (PPSMV-II) were found to be associated with sterility mosaic disease (SMD) of pigeonpea [Cajanus cajan (L.) Millsp.]. The host range of both these viruses and their vector are narrow, confined to Nicotiana benthamiana identified through mechanical transmission, and to Phaseolus vulgaris cvs. Top Crop, Kintoki, and Bountiful (F: Fabaceae) through mite transmission. A weed host Chrozophora rottleri (F: Euphorbiaceae) was also infected and tested positive for both the viruses in RT-PCR. Among the wild Cajanus species tested, Cajanus platycarpus accessions 15661, 15668, and 15671, and Cajanus scarabaeoides accessions 15683, 15686, and 15922 were infected by both the viruses and mite vector suggesting possible sources of SMD inoculum. Though accession 15666 of C. platycarpus, 15696 of C. scarabaeoides, and 15639 of Cajanus lanceolatus were infected by both the viruses, no mite infestation was observed on them. Phylogenetic analysis of nucleotide sequences of RNA-1 and RNA-2 of PPSMV-I and PPSMV-II isolates in southern India revealed significant divergence especially PPSMV-II, which is closely related to the Fig mosaic virus (FMV) than PPSMV-I. In multilocation testing of pigeonpea genotypes for their broad-based resistance to SMD for two consecutive years, genotypes ICPL-16086 and ICPL-16087 showed resistance reaction (<10% incidence) in all three locations studied. Overall, the present study gives a clear idea about the host range of PPSMV-I and PPSMV-II, their molecular relationship, and sources of resistance. This information is critical for the development of reliable diagnostic tools and improved disease management strategies.
| Item Type: | Article |
|---|---|
| Divisions: | Research Program : Asia |
| CRP: | UNSPECIFIED |
| Uncontrolled Keywords: | Cajanus cajan, SMD, PPSMV-I, PPSMV-II, host-range, host-plant resistance (HPR) |
| Subjects: | Mandate crops > Pigeonpea Others > Plant Pathology |
| Depositing User: | Mr Nagaraju T |
| Date Deposited: | 14 Mar 2024 03:34 |
| Last Modified: | 14 Mar 2024 03:34 |
| URI: | http://oar.icrisat.org/id/eprint/12576 |
| Official URL: | https://www.frontiersin.org/journals/microbiology/... |
| Projects: | UNSPECIFIED |
| Funders: | UNSPECIFIED |
| Acknowledgement: | This work has been undertaken as part of the CGIAR Research Program—Grain Legumes and Dryland Cereals (CRP-GLDC) of ICRISAT. We thank the Central Tobacco Research Institute (CTRI), Rajamahendravaram, Andhra Pradesh, India, for providing the Nicotiana species seed material. We are also thankful to C. V. Sameer Kumar and Anupama Hingane for providing the seeds of pigeonpea advanced breeding lines, and Vijay Krishna Kumar Kotamraju of the Acharya N. G. Ranga Agricultural University for critical reading of this manuscript and suggestions to improve it. BS is thankful to the University Grants Commission (UGC) for providing research fellowships (Junior Research Fellowship and Senior Research Fellowship). |
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