Louis, Y and Moses, M and Michael, C and Davis, G and Vicki, M and Abel, S and Lawrent, P and Daimon, K and Fatsani, K and Frank, T and Peter, S and Changadeya, W (2023) Phenotypic variation and genotypic diversity of Eleusine coracana L. Gaertn (finger millet) germplasm collection in Malawi. Genetic Resources and Crop Evolution, 70. pp. 2411-2424. ISSN 1573-5109
Full text not available from this repository. (Request a copy)Abstract
Eleusine coracana L. Gaertn has remained neglected and underutilized in Malawi partly due to limited research that includes crop improvement. Any effort to exploit the genetic potential of the available genetic variability could lead to identification of potential germplasm for improved varieties. This study characterised agro-morphological traits and identified genetic diversity amongst the germplasm collections using Kompetitive Allele Specifc PCR (KASP) single nucleotide polymorphism (SNP) markers to identify unique traits used for germplasm discrimination for seed production and crop improvement. A total of forty germplasm collections were tested at Crops and Soil Sciences Department's research farm of Lilongwe University of Agriculture and Natural Resources (LUANAR). The experiment was laid out in an alpha lattice design. From forty germplasm collections, 188 germplasm collection samples were selected for deoxyribonucleic acid (DNA) extraction. Phenotypic data on agro-morphological traits were collected and subjected to multivariate analysis. Genotypic data was subjected to analysis of molecular variance (AMOVA) in R software environment (R Core Team, 2021). Three principal components (PC1, PC2 and PC3) explained 42% of the total phenotypic variation among the germplasm collection while AMOVA revealed a 48% genotyping variation within and 53% genotypic variation among the germplasm collection. Cluster analysis grouped the 188 genotyped germplasm collection into 6 clusters. Phenotypic variation and genotypic diversity exist in finger millet which aids in the discrimination of the germplasm collection in seed production and in a breeding program.
| Item Type: | Article |
|---|---|
| Divisions: | Research Program : East & Southern Africa |
| CRP: | UNSPECIFIED |
| Uncontrolled Keywords: | Finger millet, Germplasm collection, Agro-morphological, Multivariate analysis, Phenotypic variation, Genotypic diversity |
| Subjects: | Mandate crops > Millets > Finger Millet Others > Germplasm Others > Malawi |
| Depositing User: | Mr Nagaraju T |
| Date Deposited: | 31 Jan 2024 09:13 |
| Last Modified: | 31 Jan 2024 09:13 |
| URI: | http://oar.icrisat.org/id/eprint/12410 |
| Official URL: | https://link.springer.com/article/10.1007/s10722-0... |
| Projects: | Research Project on Seed Systems Grant |
| Funders: | The McKnight foundation Collaborative Crop Research Program (CCRP) |
| Acknowledgement: | The author are thankful to the supervisory committee; Dr. Michael Chipeta (Major supervisor), Dr. Abel Sefasi, Associate Professor Wisdom Changadeya and Professor Moses F. A. Maliro for their technical support the time of project implementation. I would also like to thank the McKnight foundation through its Collaborative Crop Research Program (CCRP) that funded the Seed Systems’ research project (Grant No. 19-265), all collaborators for technical support at the time of project implementation. I would also like to thanks Mr. Wilson Kapyira and Mr. Peterson Nachanje for timely provision of resources. The author would also like to acknowledge Ric Core and the entire CCRP team for their technical support during data processing and analysis. The author would also like to acknowledge Excellence in Breeding (EIB) for provision of E. coracana L. Gaertn KASP SNP markers used in this study and not forgetting farmers who provided the germplasms used in this study. |
| Links: |
Actions (login required)
 |
View Item |
Altmetric
Altmetric