Patil, B and Hegde, V and Sridhara, S and Pandian, R T P and Thube, S H and Palliath, G K and Gangurde, S S and Jha, P K (2022) Multigene phylogeny and haplotype analysis reveals predominance of oomycetous fungus, Phytophthora meadii (McRae) associated with fruit rot disease of arecanut in India. Saudi Journal of Biological Sciences (TSI), 29. pp. 1-16. ISSN 1319-562X
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Abstract
An oomycetous fungus Phytophthora causing fruit rot is the most devastating disease of arecanut in different agro-climatic zones of Karnataka with varied climatic profiles. The main aim of this investigation was to characterize the geo-distant Phytophthora populations infecting arecanut using robust morphological, multi-gene phylogeny and haplotype analysis. A total of 48 geo-distant fruit rot infected samples were collected during the South-West monsoon of 2017–19. Pure culture of the suspected pathogen was isolated from the infected nuts and pathogenic ability was confirmed and characterized. Colony morphology revealed typical whitish mycelium with stellate or petalloid pattern and appearance with torulose hyphae. Sporangia were caducous, semipapillate or papillate, globose, ellipsoid or ovoid-obpyriform in shape and sporangiophores were irregularly branched or simple sympodial in nature. Subsequent multi-gene phylogeny (ITS, β-tub, TEF-1α and Cox-II) and sequence analysis confirmed the identity of oomycete as Phytophthora meadii which is predominant across the regions studied. We identified 49 haplotypes representing the higher haplotype diversity with varying relative haplotype frequency. Comprehensive study confirmed the existence of substantial variability among geo-distant populations (n = 48) of P. meadii. The knowledge on population dynamics of the pathogen causing fruit rot of arecanut generated from this investigation would aid in developing appropriate disease management strategies to curtail its further occurrence and spread in arecanut ecosystem.
| Item Type: | Article |
|---|---|
| Divisions: | Center of Excellence in Genomics and Systems Biology |
| CRP: | UNSPECIFIED |
| Uncontrolled Keywords: | Morphology, Multi-gene phylogeny, Haplotypes, Arecanut, P. meadii, Characterization |
| Subjects: | Others > Genetics and Genomics |
| Depositing User: | Mr Nagaraju T |
| Date Deposited: | 11 Jan 2024 09:25 |
| Last Modified: | 11 Jan 2024 09:25 |
| URI: | http://oar.icrisat.org/id/eprint/12352 |
| Official URL: | https://www.sciencedirect.com/science/article/pii/... |
| Projects: | UNSPECIFIED |
| Funders: | UNSPECIFIED |
| Acknowledgement: | This study was part of the Ph.D. thesis of first author and thankful to University of Agricultural and Horticultural Sciences, Shivamogga, Karnataka for providing required financial assistance and collaborative institute ICAR-Central Plantation Crops Research Institute, Kasaragod, Kerala, India for technical support, consultative facility. A help rendered by the scientist Dr. M. K. Rajesh for his criticisms, suggestions and betterment of manuscript is greatly acknowledged. |
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