Innovation in the Breeding of Common Bean Through a Combined Approach of in vitro Regeneration and Machine Learning Algorithms

Aasim, M and Katirci, R and Baloch, F S and Mustafa, Z and Bakhsh, A and Nadeem, M A and Ali, S A and Hatipoğlu, R and Çiftçi, V and Habyarimana, E and Karaköy, T and Chung, Y S (2022) Innovation in the Breeding of Common Bean Through a Combined Approach of in vitro Regeneration and Machine Learning Algorithms. Frontiers in Genetics (TSI), 13. pp. 1-13. ISSN 1664-8021

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Abstract

Common bean is considered a recalcitrant crop for in vitro regeneration and needs a repeatable and efficient in vitro regeneration protocol for its improvement through biotechnological approaches. In this study, the establishment of efficient and reproducible in vitro regeneration followed by predicting and optimizing through machine learning (ML) models, such as artificial neural network algorithms, was performed. Mature embryos of common bean were pretreated with 5, 10, and 20 mg/L benzylaminopurine (BAP) for 20 days followed by isolation of plumular apice for in vitro regeneration and cultured on a post-treatment medium containing 0.25, 0.50, 1.0, and 1.50 mg/L BAP for 8 weeks. Plumular apice explants pretreated with 20 mg/L BAP exerted a negative impact and resulted in minimum shoot regeneration frequency and shoot count, but produced longer shoots. All output variables (shoot regeneration frequency, shoot counts, and shoot length) increased significantly with the enhancement of BAP concentration in the post-treatment medium. Interaction of the pretreatment × post-treatment medium revealed the need for a specific combination for inducing a high shoot regeneration frequency. Higher shoot count and shoot length were achieved from the interaction of 5 mg/L BAP × 1.00 mg/L BAP followed by 10 mg/L BAP × 1.50 mg/L BAP and 20 mg/L BAP × 1.50 mg/L BAP. The evaluation of data through ML models revealed that R2 values ranged from 0.32 to 0.58 (regeneration), 0.01 to 0.22 (shoot counts), and 0.18 to 0.48 (shoot length). On the other hand, the mean squared error values ranged from 0.0596 to 0.0965 for shoot regeneration, 0.0327 to 0.0412 for shoot count, and 0.0258 to 0.0404 for shoot length from all ML models. Among the utilized models, the multilayer perceptron model provided a better prediction and optimization for all output variables, compared to other models. The achieved results can be employed for the prediction and optimization of plant tissue culture protocols used for biotechnological approaches in a breeding program of common beans.

Item Type:	Article
Divisions:	Global Research Program - Accelerated Crop Improvement
CRP:	UNSPECIFIED
Uncontrolled Keywords:	machine learning algorithms, artificial neural network, in vitro regeneration, plumular apices, coefficient of determination, mean squared error
Subjects:	Others > Plant Breeding Others > Genetics and Genomics
Depositing User:	Mr Nagaraju T
Date Deposited:	15 Nov 2023 10:42
Last Modified:	15 Nov 2023 10:42
URI:	http://oar.icrisat.org/id/eprint/12302
Official URL:	https://www.frontiersin.org/articles/10.3389/fgene...
Projects:	UNSPECIFIED
Funders:	National Research Foundation of Korea (NRF), funded by the Ministry of Education
Acknowledgement:	UNSPECIFIED
Links:	Google Scholar

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