Singh, S and Sharma, R and Nepolean, T and Nayak, S N and Pushpavathi, B and Khan, A W and Srivastava, R K and Varshney, R K (2022) Identification of genes controlling compatible and incompatible reactions of pearl millet (Pennisetum glaucum) against blast (Magnaporthe grisea) pathogen through RNA-Seq. Frontiers in Plant Science (TSI), 13. 01-14. ISSN 1664-462X
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Abstract
Blast [Magnaporthe grisea (Herbert) Barr] is an economically important disease in Asian pearl millet production ecologies. The recurrent occurrence of blast in the past one decade has caused enormous strain on grain and forage production. Identification of resistance genes is an important step to develop durable varieties. The present study is the first attempt to use RNA-Seq to investigate the transcript dynamics in a pearl millet inbred ICMB 93333, which had a unique differential reaction to two isolates—Pg 45 (avirulent) and Pg 174 (virulent) of M. grisea. The inbred was inoculated by both isolates and samples taken at six different time intervals for genome-wide RNA-Seq experiment. The transcriptome results revealed the differential expression of more than 2,300 genes. The time-specific comparison showed activation or repression of specific genes in various pathways. Genes and transcriptions factors related to pathogenesis-related proteins, reactive oxygen species generating and its scavenging genes, cell wall defense, primary and secondary metabolic pathways, and signaling pathways were identified by comparing the host-plant compatible and incompatible interactions. The genes identified from this experiment could be useful to understand the host-plant resistance and design novel strategies to manage blast disease in pearl millet.
| Item Type: | Article |
|---|---|
| Divisions: | Research Program : Asia |
| CRP: | CGIAR Research Program on Grain Legumes and Dryland Cereals (GLDC) |
| Uncontrolled Keywords: | differential reaction, blast, transcriptome, RNA-Seq, resistance |
| Subjects: | Mandate crops > Millets > Pearl Millet Others > Genetics and Genomics Others > Plant Pathology |
| Depositing User: | Mr Nagaraju T |
| Date Deposited: | 01 Nov 2023 09:41 |
| Last Modified: | 01 Nov 2023 09:41 |
| URI: | http://oar.icrisat.org/id/eprint/12264 |
| Official URL: | https://www.frontiersin.org/articles/10.3389/fpls.... |
| Projects: | UNSPECIFIED |
| Funders: | CGIAR Research Program on Grain Legumes and Dryland Cereals (CRP-GLDC) |
| Acknowledgement: | The authors are thankful to the CGIAR Research Program on Grain Legumes and Dryland Cereals (CRP-GLDC) and Pearl Millet Hybrid Parents Research Consortium (PMHPRC) for the financial support. |
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