Saroha, A and Gomashe, S S and Kaur, V and Pal, D and Ujjainwal, S and Aravind, J and Singh, M and Rajkumar, S and Singh, K and Kumar, A and Wankhede, D P (2023) Genetic dissection of thousand-seed weight in linseed (Linum usitatissimum L.) using multi-locus genome-wide association study. Frontiers in Plant Science (TSI), 14. pp. 1-15. ISSN 1664-462X
Full text not available from this repository. (Request a copy)Abstract
Flaxseed/linseed is an important oilseed crop having applications in the food, nutraceutical, and paint industry. Seed weight is one of the most crucial determinants of seed yield in linseed. Here, quantitative trait nucleotides (QTNs) associated with thousand-seed weight (TSW) have been identified using multi-locus genome-wide association study (ML-GWAS). Field evaluation was carried out in five environments in multi-year-location trials. SNP genotyping information of the AM panel of 131 accessions comprising 68,925 SNPs was employed for ML-GWAS. From the six ML-GWAS methods employed, five methods helped identify a total of 84 unique significant QTNs for TSW. QTNs identified in ≥ 2 methods/environments were designated as stable QTNs. Accordingly, 30 stable QTNs have been identified for TSW accounting up to 38.65% trait variation. Alleles with positive effect on trait were analyzed for 12 strong QTNs with r2 ≥ 10.00%, which showed significant association of specific alleles with higher trait value in three or more environments. A total of 23 candidate genes have been identified for TSW, which included B3 domaincontaining transcription factor, SUMO-activating enzyme, protein SCARECROW, shaggy-related protein kinase/BIN2, ANTIAUXIN-RESISTANT 3, RING-type E3 ubiquitin transferase E4, auxin response factors, WRKY transcription factor, and CBS domain-containing protein. In silico expression analysis of candidate genes was performed to validate their possible role in different stages of seed development process. The results from this study provide significant insight and elevate our understanding on genetic architecture of TSW trait in linseed.
| Item Type: | Article |
|---|---|
| Divisions: | Genebank |
| CRP: | UNSPECIFIED |
| Uncontrolled Keywords: | linseed, flaxseed, seed weight, genome-wide association studies, quantitative trait nucleotides, candidate genes |
| Subjects: | Others > Genetics and Genomics Others > Seeds/Seed Bank |
| Depositing User: | Mr Nagaraju T |
| Date Deposited: | 19 Oct 2023 04:32 |
| Last Modified: | 19 Oct 2023 04:32 |
| URI: | http://oar.icrisat.org/id/eprint/12223 |
| Official URL: | https://www.frontiersin.org/articles/10.3389/fpls.... |
| Projects: | UNSPECIFIED |
| Funders: | UNSPECIFIED |
| Acknowledgement: | Authors acknowledge funding to project (ECR/2017/001280) from Science & Engineering Research Board (SERB), and Department of Biotechnology (DBT), Government of India for project (No. BT/Ag/Network/Linseed/2019-20). Authors thank Director, ICAR-NBPGR and Heads, DGR, DGE for research facilities and facilitation. |
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