Jadesha, G and Sharma, M and Reddy, P N (2022) Laboratory protocol for induction of sporulation in Phytophthora cajani causing phytophthora blight in pigeonpea. Legume Research (TSI), 45 (11). pp. 1445-1451. ISSN 0976-0571
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Background Phytophthora blight (PB), caused by Phytophthora cajani is a prominent disease in the low laying areas combined with intermittent rainfall. Induction of zoospores and sporangia of P. cajani in culture plate is troublesome and also limited information is available on the protocol for sporulation of sporangia and zoospores in the laboratory, The study developed and validated the protocol for induction of sporangia and zoospore of P. cajani. Methods The Protocol using 5–7 days old culture, diluted tomato extract broth and other culture conditions like the temperature of 30°C, alternate 12 hours of fluorescent light (2000 Lx) and dark can induce abundant sporangia and zoospores in vitro. Result The study developed and validated the new protocol for uniform and profuse induction of sporangia and zoospores of P. cajani. Diplanetism mechanism of Phytophthora spp. was recorded with P. cajani the findings are the first of its kind. Additionally, the study revealed the concentration of 1×10−5 zoospores/ml is optimum to develop the infection in plants with the shortest incubation period of 24 hours.
| Item Type: | Article |
|---|---|
| Divisions: | Research Program : Asia |
| CRP: | UNSPECIFIED |
| Uncontrolled Keywords: | Diplanetism, Phytophthora, Pigeonpea blight, Sporangia, Sporulation, Zoospores |
| Subjects: | Mandate crops > Pigeonpea Others > Plant Pathology Others > Plant Disease |
| Depositing User: | Mr Nagaraju T |
| Date Deposited: | 29 Sep 2023 04:39 |
| Last Modified: | 29 Sep 2023 04:39 |
| URI: | http://oar.icrisat.org/id/eprint/12194 |
| Official URL: | https://www.indianjournals.com/ijor.aspx?target=ij... |
| Projects: | UNSPECIFIED |
| Funders: | UNSPECIFIED |
| Acknowledgement: | The funding support from Indian Council of Agricultural Research (ICAR), New Delhi, is gratefully acknowledged. |
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