Genomic, morphological, and biochemical analyses of a multi‑metal resistant but multi‑drug susceptible strain of Bordetella petrii from hospital soil

Halder, U and Biswas, R and Kabiraj, A and Deora, R and Let, M and Roy, R Kr and Chitikineni, A and Majhi, K and Sarkar, S and Dutta, B and Laha, A and Datta, A and Khan, D and Varshney, R K and Saha, D and Chattopadhyay, S and Bandopadhyay, R (2022) Genomic, morphological, and biochemical analyses of a multi‑metal resistant but multi‑drug susceptible strain of Bordetella petrii from hospital soil. Scientific Reports (TSI), 12. 01-13. ISSN 2045-2322

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Abstract

Contamination of soil by antibiotics and heavy metals originating from hospital facilities has emerged as a major cause for the development of resistant microbes. We collected soil samples surrounding a hospital effluent and measured the resistance of bacterial isolates against multiple antibiotics and heavy metals. One strain BMCSI 3 was found to be sensitive to all tested antibiotics. However, it was resistant to many heavy metals and metalloids like cadmium, chromium, copper, mercury, arsenic, and others. This strain was motile and potentially spore-forming. Whole-genome shotgun assembly of BMCSI 3 produced 4.95 Mb genome with 4,638 protein-coding genes. The taxonomic and phylogenetic analysis revealed it, to be a Bordetella petrii strain. Multiple genomic islands carrying mobile genetic elements; coding for heavy metal resistant genes, response regulators or transcription factors, transporters, and multi-drug efflux pumps were identified from the genome. A comparative genomic analysis of BMCSI 3 with annotated genomes of other free-living B. petrii revealed the presence of multiple transposable elements and several genes involved in stress response and metabolism. This study provides insights into how genomic reorganization and plasticity results in evolution of heavy metals resistance by acquiring genes from its natural environment.

Item Type:	Article
Divisions:	Center of Excellence in Genomics and Systems Biology
CRP:	UNSPECIFIED
Uncontrolled Keywords:	Genomic, morphological, Bordetella petrii, soil
Subjects:	Others > Soil Others > Genetics and Genomics
Depositing User:	Mr Nagaraju T
Date Deposited:	04 Sep 2023 08:50
Last Modified:	04 Sep 2023 08:50
URI:	http://oar.icrisat.org/id/eprint/12171
Official URL:	https://www.nature.com/articles/s41598-022-12435-7
Projects:	UNSPECIFIED
Funders:	UNSPECIFIED
Acknowledgement:	UH is thankful to SRF (State Fund) fellowship [Fc (Sc.)/RS/SF/ BOT./2017-18/22], AK is thankful to DHESTBT (WB-DBT), Memo No. [Fc (Sc.)/RS/SF/ BOT./2017-18/22], ML and RKR are thankful to UGC JRF for financial assistance to conduct this research work. AD is thankful to DS Kothari Fellowship.
Links:	Google Scholar

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