Detection of Salmonella by Surface Plasmon Resonance

Barlen, B and Mazumdar, S D and Lezrich, O and Kämpfer, P and Keusgen, M (2007) Detection of Salmonella by Surface Plasmon Resonance. Sensors, 7 (8). pp. 1427-1446. ISSN 1424-8220

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Abstract

This study explores the possibility of simultaneous and specific detection of Salmonella serovars by surface plasmon resonance (SPR). The Plasmonic® SPR device was used to develop this rapid assay. The sandwich immunoassay involves the use of a polyclonal anti-Salmonella antibody to simultaneous capture multiple Salmonella serovars present in a sample. This is followed by specific detection of the captured serovars using O-specific anti-Salmonella antibodies. Milk spiked with Salmonella Typhimurium and Salmonella Enteritidis was used as a model system to establish the assay. The assay was further extended to sequentially differentiate between the two Salmonella serovars on a single SPR chip in a single channel. The assay was proved to work without any additional dilution or clean-up steps. The sample volume requirement for the assay is only 10 μL. The lower limits of detection for Salmonella Typhimurium and Salmonella Enteritidis were 2.50×105 cells mL-1 and 2.50×108 cells mL-1, respectively.

Item Type:	Article
Divisions:	Global Research Program - Accelerated Crop Improvement
CRP:	UNSPECIFIED
Uncontrolled Keywords:	Salmonella serovars, Lipopolysaccharide (LPS), O-specific antibody, Sequential detection, Plasmonic surface plasmon resonance (SPR) device
Subjects:	Others > Food and Nutrition
Depositing User:	Mr Arun S
Date Deposited:	20 Jan 2022 15:16
Last Modified:	20 Jan 2022 15:16
URI:	http://oar.icrisat.org/id/eprint/11948
Official URL:	https://doi.org/10.3390/s7081427
Projects:	UNSPECIFIED
Funders:	UNSPECIFIED
Acknowledgement:	The authors would like to thank Mrs Gundula Will for her help in preparing the bacterial samples, and also Mr Floris van Elsäcker for arranging fresh milk samples from local sources.
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