Genome‐wide miRNAs profiles of pearl millet under contrasting high vapor pressure deficit reveal their functional roles in drought stress adaptations

Palakolanu, S R and Gupta, S and Yeshvekar, R K and Chakravartty, N and Kaliamoorthy, S and Shankhapal, A R and Vempati, A S and Kuriakose, B and Lekkala, S P and Philip, M and Perumal, R C and Lachagari, V B R and Bhatnagar‐Mathur, P (2021) Genome‐wide miRNAs profiles of pearl millet under contrasting high vapor pressure deficit reveal their functional roles in drought stress adaptations. Physiologia Plantarum (TSI). pp. 1-17. ISSN 0031-9317

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Abstract

Pearl millet (Pennisetum glaucum [L.] R. Br.) is an important crop capable of growing in harsh and marginal environments, with the highest degree of tolerance to drought and heat stresses among cereals. Diverse germplasm of pearl millet shows a significant phenotypic variation in response to abiotic stresses, making it a unique model to study the mechanisms responsible for stress mitigation. The present study focuses on identifying the physiological response of two pearl millet high-resolution cross (HRC) genotypes, ICMR 1122 and ICMR 1152, in response to low and high vapor pressure deficit (VPD). Under high VPD conditions, ICMR 1152 exhibited a lower transpiration rate (Tr), higher transpiration efficiency, and lower root sap exudation than ICMR 1122. Further, Pg-miRNAs expressed in the contrasting genotypes under low and high VPD conditions were identified by deep sequencing analysis. A total of 116 known and 61 novel Pg-miRNAs were identified from ICMR 1152, while 26 known and six novel Pg-miRNAs were identified from ICMR 1122 genotypes, respectively. While Pg-miR165, 168, 170, and 319 families exhibited significant differential expression under low and high VPD conditions in both genotypes, ICMR 1152 showed abundant expression of Pg-miR167, Pg-miR172, Pg-miR396 PgmiR399, Pg-miR862, Pg-miR868, Pg-miR950, Pg-miR5054, and Pg-miR7527 indicating their direct and indirect role in root physiology and abiotic stress responses. Drought responsive Pg-miRNA targets showed upregulation in response to high VPD stress, further narrowing down the miRNAs involved in regulation of drought tolerance in pearl millet.

Item Type:	Article
Divisions:	Global Research Program - Accelerated Crop Improvement
CRP:	CGIAR Research Program on Grain Legumes and Dryland Cereals (GLDC)
Uncontrolled Keywords:	Pearl Millet, Drought Tolerance, Cereals
Subjects:	Others > Drought Tolerance Mandate crops > Millets > Pearl Millet
Depositing User:	Mr Arun S
Date Deposited:	04 Nov 2021 13:07
Last Modified:	04 Nov 2021 13:07
URI:	http://oar.icrisat.org/id/eprint/11911
Official URL:	https://doi.org/10.1111/ppl.13521
Projects:	UNSPECIFIED
Funders:	UNSPECIFIED
Acknowledgement:	This work was undertaken as part of the CGIAR Research Program on Grain Legumes & Dryland Cereals (CRP-GLDC). This study was supported by Science and Engineering Research Board, Department of Science and Technology, Government of India for the core grant research (CRG) grant file no. DST No: EMR/20l6/006726.
Links:	Google Scholar

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