Genome sequencing and comparative genomic analysis of highly and weakly aggressive strains of Sclerotium rolfsii, the causal agent of peanut stem rot

    Yan, L and Wang, Z and Song, W and Fan, P and Kang, Y and Lei, Y and Wan, L and Huai, D and Chen, Y and Wang, X and Sudini, H and Liao, B (2021) Genome sequencing and comparative genomic analysis of highly and weakly aggressive strains of Sclerotium rolfsii, the causal agent of peanut stem rot. BMC Genomics (TSI), 22 (1). pp. 1-15. ISSN 1471-2164

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    Abstract

    Background: Stem rot caused by Sclerotium rolfsii is a very important soil-borne disease of peanut. S. rolfsii is a necrotrophic plant pathogenic fungus with an extensive host range and worldwide distribution. It can infect peanut stems, roots, pegs and pods, leading to varied yield losses. S. rolfsii strains GP3 and ZY collected from peanut in different provinces of China exhibited a significant difference in aggressiveness on peanut plants by artificial inoculation test. In this study, de-novo genome sequencing of these two distinct strains was performed aiming to reveal the genomic basis of difference in aggressiveness. Results: Scleotium rolfsii strains GP3 and ZY, with weak and high aggressiveness on peanut plants, exhibited similar growth rate and oxalic acid production in laboratory. The genomes of S. rolfsii strains GP3 and ZY were sequenced by Pacbio long read technology and exhibited 70.51 Mb and 70.61 Mb, with contigs of 27 and 23, and encoded 17, 097 and 16,743 gene models, respectively. Comparative genomic analysis revealed that the pathogenicity-related gene repertoires, which might be associated with aggressiveness, differed between GP3 and ZY. There were 58 and 45 unique pathogen-host interaction (PHI) genes in GP3 and ZY, respectively. The ZY strain had more carbohydrateactive enzymes (CAZymes) in its secretome than GP3, especially in the glycoside hydrolase family (GH), the carbohydrate esterase family (CBM), and the polysaccharide lyase family (PL). GP3 and ZY also had different effector candidates and putative secondary metabolite synthetic gene clusters. These results indicated that differences in PHI, secreted CAZymes, effectors and secondary metabolites may play important roles in aggressive difference between these two strains. Conclusions: The data provided a further understanding of the S. rolfsii genome. Genomic comparison provided clues to the difference in aggressiveness of S. rolfsii strains.

    Item Type: Article
    Divisions: Research Program : Asia
    CRP: UNSPECIFIED
    Uncontrolled Keywords: Comparative genomic analysis, PacBio sequel sequencing, Pathogenesis-related genes, Sclerotium rolfsii
    Subjects: Others > Genetics and Genomics
    Others > Plant Disease
    Depositing User: Mr Arun S
    Date Deposited: 27 May 2021 14:36
    Last Modified: 27 May 2021 14:36
    URI: http://oar.icrisat.org/id/eprint/11825
    Official URL: https://doi.org/10.1186/s12864-021-07534-0
    Projects: UNSPECIFIED
    Funders: National Natural Science Foundation of China
    Acknowledgement: We thank Dr. Huizhang Zhao for analyzing CAZymes of S. rolfsii ZY and GP3.
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