Comparative Transcriptome Analysis Identified Candidate Genes for Late Leaf Spot Resistance and Cause of Defoliation in Groundnut

    Gangurde, S S and Nayak, S N and Joshi, P and Purohit, S and Sudini, H K and Chitikineni, A and Hong, Y and Guo, B and Chen, X and Pandey, M K and Varshney, R K (2021) Comparative Transcriptome Analysis Identified Candidate Genes for Late Leaf Spot Resistance and Cause of Defoliation in Groundnut. International Journal of Molecular Sciences (TSI), 22 (4491). pp. 1-23. ISSN 1422-0067

    [img] PDF - Published Version
    Download (6MB)

    Abstract

    Late leaf spot (LLS) caused by fungus Nothopassalora personata in groundnut is responsible for up to 50% yield loss. To dissect the complex nature of LLS resistance, comparative transcriptome analysis was performed using resistant (GPBD 4), susceptible (TAG 24) and a resistant introgression line (ICGV 13208) and identified a total of 12,164 and 9954 DEGs (differentially expressed genes) respectively in A- and B-subgenomes of tetraploid groundnut. There were 135 and 136 unique pathways triggered in A- and B-subgenomes, respectively, upon N. personata infection. Highly upregulated putative disease resistance genes, an RPP-13 like (Aradu.P20JR) and a NBS-LRR (Aradu.Z87JB) were identified on chromosome A02 and A03, respectively, for LLS resistance. Mildew resistance Locus (MLOs)-like proteins, heavy metal transport proteins, and ubiquitin protein ligase showed trend of upregulation in susceptible genotypes, while tetratricopeptide repeats (TPR), pentatricopeptide repeat (PPR), chitinases, glutathione S-transferases, purple acid phosphatases showed upregulation in resistant genotypes. However, the highly expressed ethylene responsive factor (ERF) and ethylene responsive nuclear protein (ERF2), and early responsive dehydration gene (ERD) might be related to the possible causes of defoliation in susceptible genotypes. The identified disease resistance genes can be deployed in genomics-assisted breeding for development of LLS resistant cultivars to reduce the yield loss in groundnut.

    Item Type: Article
    Divisions: Research Program : Asia
    Research Program : Genetic Gains
    CRP: CGIAR Research Program on Grain Legumes and Dryland Cereals (GLDC)
    Uncontrolled Keywords: Late leaf spot, Arachis hypogaea, Nothopassalora personata, RNA-seq, Differentially expressed genes, Pathway analysis, Defoliation, Peanut
    Subjects: Mandate crops > Groundnut
    Others > Genetics and Genomics
    Others > Plant Disease
    Depositing User: Mr Arun S
    Date Deposited: 27 May 2021 07:28
    Last Modified: 27 May 2021 07:28
    URI: http://oar.icrisat.org/id/eprint/11822
    Official URL: https://doi.org/10.3390/ijms22094491
    Projects: UNSPECIFIED
    Funders: UNSPECIFIED
    Acknowledgement: The authors are thankful to National Agricultural Science Fund (NASF) of Indian Council of Agricultural Research (ICAR), Department of Biotechnology (DBT), India and Bill and Melinda Gates Foundation (BMGF), USA for partial financial assistance. Sunil S. Gangurde acknowledges the Department of Science and Technology (DST), Govt. of India for the award of the INSPIRE fellowship during a PhD. Authors are grateful to Ashwin Jayale and Prasad Bajaj for their technical help. The work reported in this article was undertaken as a part of the CGIAR Research Program on Grain Legumes and Dryland Cereals (GLDC). ICRISAT is a member of the CGIAR.
    Links:
    View Statistics

    Actions (login required)

    View Item View Item
    Altmetric
    OAR@ICRISAT is powered by EPrints 3 which is developed by the School of Electronics and Computer Science at the University of Southampton. More information and software credits.