Introgression of “ QTL‐hotspot ” region enhances drought tolerance and grain yield in three elite chickpea cultivars

Bharadwaj, C and Tripathi, S and Soren, K R and Thudi, M and Singh, R K and Sheoran, S and Roorkiwal, M and Patil, B S and Chitikineni, A and Palakurthi, R and Vemula, A K and Rathore, A and Kumar, Y and Chaturvedi, S K and Mondal, B and Shanmugavadivel, P S and Srivastava, A K and Dixit, G P and Singh, N P and Varshney, R K (2021) Introgression of “ QTL‐hotspot ” region enhances drought tolerance and grain yield in three elite chickpea cultivars. The Plant Genome (TSI), 14 (1). pp. 1-14. ISSN 1940-3372

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Abstract

With an aim of enhancing drought tolerance using a marker-assisted backcrossing (MABC) approach, we introgressed the “QTL-hotspot” region from ICC 4958 accession that harbors quantitative trait loci (QTLs) for several drought-tolerance related traits into three elite Indian chickpea (Cicer arietinum L.) cultivars: Pusa 372, Pusa 362, and DCP 92-3. Of eight simple sequence repeat (SSR) markers in the QTLhotspot region, two to three polymorphic markers were used for foreground selection with respective cross-combinations. A total of 47, 53, and 46 SSRs were used for background selection in case of introgression lines (ILs) developed in genetic backgrounds of Pusa 372, Pusa 362, and DCP 92-3, respectively. In total, 61 ILs (20 BC3F3 in Pusa 372; 20 BC2F3 in Pusa 362, and 21 BC3F3 in DCP 92-3), with >90% recurrent parent genome recovery were developed. Six improved lines in different genetic backgrounds (e.g. BGM 10216 in Pusa 372; BG 3097 and BG 4005 in Pusa 362; IPC(L4-14), IPC(L4-16), and IPC(L19-1) in DCP 92-3) showed better performance than their respective recurrent parents. BGM 10216, with 16% yield gain over Pusa 372, has been released as Pusa Chickpea 10216 by the Central Sub-Committees on Crop Standards, Notification and Release of Varieties of Agricultural Crops,Ministry of Agriculture and Farmers Welfare, Government of India, for commercial cultivation in India. In summary, this study reports introgression of the QTL-hotspot for enhancing yield under rainfed conditions, development of several introgression lines, and release of Pusa Chickpea 10216 developed through molecular breeding in India.

Item Type:	Article
Divisions:	Research Program : Genetic Gains Statistics, Bio-Informatics & Data Management
CRP:	UNSPECIFIED
Agro Tags:	Drought Tolerance, Chickpea
Subjects:	Others > Crop Yield Mandate crops > Chickpea
Depositing User:	Mr Arun S
Date Deposited:	12 May 2021 14:49
Last Modified:	12 May 2021 14:49
URI:	http://oar.icrisat.org/id/eprint/11794
Official URL:	https://doi.org/10.1002/tpg2.20076
Projects:	UNSPECIFIED
Funders:	UNSPECIFIED
Acknowledgement:	Authors greatly acknowledge the Department of Biotechnology of Ministry of Science and Technology and Department of Agriculture & Cooperation of Ministry of Agriculture and Farmers Welfare of Government of India, and partially to Bill & Melinda Gates Foundation for financial support to this study
Links:	Google Scholar

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