An update and perspectives on the use of promoters in plant genetic engineering

Kummari, D and Palakolanu, S R and Kishor, P B K and Bhatnagar-Mathur, P and Singam, P and Vadez, V and Sharma, K K (2020) An update and perspectives on the use of promoters in plant genetic engineering. Journal of Biosciences (TSI), 45 (1). pp. 1-24. ISSN 0250-5991

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Abstract

Genetically engineered plants have varied applications in agriculture for enhancing the values of food and feed. Genetic engineering aims to introduce selected genetic regions with desirable traits into target plants for both spatial and temporal expressions. Promoters are the key elements responsible for regulating gene expressions by modulating the transcription factors (TFs) through recognition of RNA polymerases. Based on their recognition and expression, RNA polymerases were categorized into RNA pol II and pol III promoters. Promoter activity and specificity are the two prime parameters in regulating the transgene expression. Since the use of constitutive promoters like Cauliflower mosaic virus (CaMV) 35S may lead to adverse effects on nontarget organisms or ecosystem, inducible/tissue specific promoters and/or the RNA pol III promoters provide myriad opportunities for gene expressions with controlled regulation and with minimum adverse effects. Besides their role in transgene expression, their influence in synthetic biology and genome editing are also discussed. This review provides an update on the importance, current prospects, and insight into the advantages and disadvantages of promoters reported thus far would help to utilize them in the endeavour to develop nutritionally and agronomically improved transgenic crops for commercialization.

Item Type:	Article
Divisions:	Research Program : Genetic Gains
CRP:	CGIAR Research Program on Grain Legumes and Dryland Cereals (GLDC)
Uncontrolled Keywords:	CaMV35S promoter, Constitutive promoters, Synthetic promoters, Genetic engineering, RNA pol II promoters, RNA pol III promoters, U3 promoter, U6 promoter
Subjects:	Others > Genetic Engineering
Depositing User:	Mr Arun S
Date Deposited:	26 Mar 2021 07:36
Last Modified:	26 Mar 2021 07:36
URI:	http://oar.icrisat.org/id/eprint/11766
Official URL:	https://doi.org/10.1007/s12038-020-00087-6
Projects:	UNSPECIFIED
Funders:	UNSPECIFIED
Acknowledgement:	PSR acknowledges financial support from the Department of Science and Technology, Govt. of India, New Delhi, for a fellowship and research grant through the INSPIRE Faculty Award No. IFALSPA-06 and Young Scientist Award SB/YS/LS-12/2013. This work was undertaken as part of the CGIAR Research Program on Grain Legumes & Dryland Cereals (CRP-GLDC).
Links:	Google Scholar

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