Genome-wide identification of meiotic recombination hot spots detected by SLAF in peanut (Arachis hypogaea L.)

Wang, X and Xu, P and Ren, Y and Yin, L and Li, S and Wang, Y and Shi, Y and Li, H and Cao, X and Chi, X and Yu, T and Pandey, M K and Varshney, R K and Yuan, M (2020) Genome-wide identification of meiotic recombination hot spots detected by SLAF in peanut (Arachis hypogaea L.). Scientific Reports (TSI), 10 (1). ISSN 2045-2322

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Abstract

Recombination hot spots (RHP), caused by meiosis, are considered to play crucial roles in improvement and domestication of crop. Cultivated peanut is one of the most important rich-source of oil and protein crops. However, no direct scale of recombination events and RHP have been estimated for peanut. To examine the scale of recombination events and RHP in peanut, a RIL population with 200 lines and a natural population with 49 cultivars were evaluated. The precise integrated map comprises 4837 SLAF markers with genetic length of 2915.46 cM and density of 1.66 markers per cM in whole genome. An average of 30.0 crossover (2.06 cMMb− 1) events was detected per RIL plant. The crossover events (CE) showed uneven distribution among B sub-genome (2.32) and A sub-genome (1.85). There were 4.34% and 7.86% of the genome contained large numbers of CE (> 50 cMMb− 1) along chromosomes in F6 and natural population, respectively. High density of CE regions called RHP, showed negative relationship to marker haplotypes conservative region but positive to heatmap of recombination. The genes located within the RHP regions by GO categories showed the responding of environmental stimuli, which suggested that recombination plays a crucial role in peanut adaptation to changing environments

Item Type:	Article
Divisions:	Research Program : Genetic Gains
CRP:	UNSPECIFIED
Uncontrolled Keywords:	Groundnut, Genomics, Genetics
Subjects:	Mandate crops > Groundnut Others > Oilseeds Others > Genetics and Genomics Others > Food and Nutrition
Depositing User:	Mr Arun S
Date Deposited:	06 Sep 2020 07:06
Last Modified:	06 Sep 2020 07:06
URI:	http://oar.icrisat.org/id/eprint/11599
Official URL:	https://doi.org/10.1038/s41598-020-70354-x
Projects:	UNSPECIFIED
Funders:	UNSPECIFIED
Acknowledgement:	This study was supported by Shandong Provincial Natural Science Foundation (ZR2019PC055; ZR2018PC023), the National Natural Science Foundation of China (31471533, 31701376; 31700553; 31801868), The National Ten Thousand Youth Talents Plan of 2014 (W02070268), China Agriculture Research System (CARS-13), The foundation of Linyi university (40618112; 40618113). Thanks to Thomas D Alcock from the Plant and Crop Sciences Division, School of Biosciences, University of Nottingham, Sutton Bonington Campus, Loughborough, LE12 5RD, United Kingdom, for English proofreading.
Links:	Google Scholar

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