Multiomics approach unravels fertility transition in a pigeonpea line for a two‐line hybrid system

Pazhamala, L T and Chaturvedi, P and Bajaj, P and Srikanth, S and Ghatak, A and Chitikineni, A and Bellaire, A and Hingane, A and Kumar, C V S and Saxena, K B and Weckwerth, W and Saxena, R K and Varshney, R K (2020) Multiomics approach unravels fertility transition in a pigeonpea line for a two‐line hybrid system. The Plant Genome (TSI), 13 (2). pp. 1-20. ISSN 1940-3372

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Abstract

Pigeonpea [Cajanus cajan (L.) Millsp.] is a pulse crop cultivated in the semi-arid regions of Asia and Africa. It is a rich source of protein and capable of alleviating malnutrition, improving soil health and the livelihoods of small-holder farmers. Hybrid breeding has provided remarkable improvements for pigeonpea productivity, but owing to a tedious and costly seed production system, an alternative two-line hybrid technology is being explored. In this regard, an environmentsensitive male sterile line has been characterized as a thermosensitive male sterile line in pigeonpea precisely responding to day temperature. The male sterile and fertile anthers from five developmental stages were studied by integrating transcriptomics, proteomics and metabolomics supported by precise phenotyping and scanning electron microscopic study. Spatio-temporal analysis of anther transcriptome and proteome revealed 17 repressed DEGs/DEPs in sterile anthers that play a critical role in normal cell wall morphogenesis and tapetal cell development. The male fertility to sterility transitionwasmainly due to a perturbation in auxin homeostasis, leading to impaired cellwallmodification and sugar transport. Limited nutrient utilization thus leads to microspore starvation in response to moderately elevated day temperature which could be restored with auxin-treatment in the male sterile line. Our findings outline a molecular mechanism that underpins fertility transition responses thereby providing a process-oriented two-line hybrid breeding framework for pigeonpea.

Item Type:	Article
Divisions:	Research Program : Genetic Gains
CRP:	CGIAR Research Program on Grain Legumes and Dryland Cereals (GLDC)
Uncontrolled Keywords:	Pigeonpea, Nutrition, Small-holder Farmer, Breeding
Subjects:	Others > Plant Breeding Mandate crops > Pigeonpea Others > Genetics and Genomics Others > Food and Nutrition
Depositing User:	Mr Arun S
Date Deposited:	04 Sep 2020 12:40
Last Modified:	04 Sep 2020 12:40
URI:	http://oar.icrisat.org/id/eprint/11582
Official URL:	https://doi.org/10.1002/tpg2.20028
Projects:	UNSPECIFIED
Funders:	UNSPECIFIED
Acknowledgement:	This study was supported by Blue Sky Research and Systems Biology Research Initiative from ICRISAT, partially funded by the US Agency for International Development (USAID), India Mission and Department of Agriculture Cooperation & FarmersWelfare, Ministry of Agriculture & FarmersWelfare, Government of India and Bill & Melinda Gates Foundation (Tropical Legumes II). Dr. Rajeev K. Varshney is also thankful to the Science & Engineering Research Board (SERB) of Department of Science & Technology (DST), Government of India, for providing the J C Bose National Fellowship (SB/S9/Z-13/2019). Dr. Arindam Ghatak was supported by a research grant provided by Austrian Science Fund (FWF, Der Wissenschaftsfonds; Grant agreement number W1257-20). We would like to gratefully acknowledge Dr. Vinay Kumar, Ms. Akanksha Kulshreshtha, Mr. M. Manikyam and Mr. Jaipal Goud for extending technical support. Thanks are due to Mr. Joseph Mathews and Mr. Anis Akhtar for assisting in growth chamber experiments. We extend our thanks to Dr. L. Krishnamurthy for providing valuable suggestions on setting up experiments under controlled plant growth conditions. This work has been undertaken as part of the CGIAR Research Program on Grain Legumes and Dryland Cereals (GLDC). ICRISAT is a member of CGIAR Consortium.
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