Afolayan, G and Aladele, S E and Deshpande, S P and Oduoye, O T and Nwosu, D J and Michael, C and Blay, E T and Danquah, E Y (2019) Marker Assisted Foreground Selection for Identification of Striga Resistant Backcross Lines in Sorghum bicolor. Covenant Journal of Physical and Life Sciences, 7 (1). pp. 29-36.
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Abstract
Striga is a major constraint to sorghum production causing high yield loss due to increasing infestation. Locally-adapted cultivars with resistant genes/QTLs could be an effective control strategy for Striga. Marker-Assisted Foreground Selection was used to select backcross lines possessing Striga resistance QTLs from N13. Marker polymorphism was conducted for the donor parent N13 and 10 recurrent parents using 10 Simple Sequence Repeat (SSR) markers. Recurrent parents with SSR alleles, polymorphic to the donor parent allele were selected. F1 lines were developed by making a cross between the selected recurrent parent and the donor. The F1 were confirmed for heterozygosity using SSR markers. Selected heterozygote F1s were backcrossed to their recurrent parent to develop backcross populations (BC1F1 and BC2F1). BC1F1 and BC2F1 populations were genotyped using SSR markers flanking the Striga resistant QTLs in N13. Forty two DANYANA-N13 BC2F1 lines (with 4 QTLs in 3 lines, 3 QTLs in 10 lines and other 28 lines having 1 to 2 QTLs) were selected for the presence of N13 QTLs. Forty three SAMSORG39-N13 BC2F1 lines (with 3 QTLs in 2 lines while 41 lines had 1 to 2 QTLs) were also selected for the presence of N13 QTLs. Although, selected lines will be genotyped for the recovery of recurrent parent background and evaluated to identify elite genotypes for possible release as varieties, the successful introgression of Striga resistance QTLs using Marker Assisted Selection suggests that in developing superior sorghum varieties, breeders could make use of molecular marker technologies to speed up breeding programmes.
| Item Type: | Article |
|---|---|
| Divisions: | Research Program : Genetic Gains |
| CRP: | UNSPECIFIED |
| Uncontrolled Keywords: | Backcross, Marker Assisted Selection, Striga, Sorghum, Simple Sequence Repeats (SSR) |
| Subjects: | Others > Cereals Mandate crops > Sorghum Others > Genetics and Genomics |
| Depositing User: | Mr Arun S |
| Date Deposited: | 12 Mar 2020 03:42 |
| Last Modified: | 12 Mar 2020 03:42 |
| URI: | http://oar.icrisat.org/id/eprint/11384 |
| Official URL: | http://journals.covenantuniversity.edu.ng/index.ph... |
| Projects: | UNSPECIFIED |
| Funders: | UNSPECIFIED |
| Acknowledgement: | The authors would like to thank the Alliance for Green Revolution in Africa (AGRA) for financial support and West Africa Centre for Crop Improvement (WACCI) for good supervision. The Institute for Agricultural Research (IAR) Nigeria is well acknowledged for supply of germplasm and International Crop Research Institute for Semi-Arid Tropics (ICRISAT), India is appreciated for genotyping platform. |
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