Steady expression of high oleic acid in peanut bred by marker-assisted backcrossing for fatty acid desaturase mutant alleles and its effect on seed germination along with other seedling traits

    Prasad, M and Bera, S K and Kamdar, J H and Kasundra, S V and Patel, S V and Jasani, M D and Maurya, A K and Dash, P and Chandrashekar, A B and Rani, K and Manivannan, N and Janila, P and Pandey, M K and Vasanthi, R P and Dobariya, K L and Radhakrishnan, T and Varshney, R K (2019) Steady expression of high oleic acid in peanut bred by marker-assisted backcrossing for fatty acid desaturase mutant alleles and its effect on seed germination along with other seedling traits. PLOS ONE (TSI), 14 (12). pp. 1-21. ISSN 1932-6203

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    Abstract

    Peanut (Arachis hypogaea L.) is an important nutrient-rich food legume and valued for its good quality cooking oil. The fatty acid content is the major determinant of the quality of the edible oil. The oils containing higher monounsaturated fatty acid are preferred for improved shelf life and potential health benefits. Therefore, a high oleic/linoleic fatty acid ratio is the target trait in an advanced breeding program. The two mutant alleles, ahFAD2A (on linkage group a09) and ahFAD2B (on linkage group b09) control fatty acid composition for higher oleic/linoleic ratio in peanut. In the present study, marker-assisted backcrossing was employed for the introgression of two FAD2 mutant alleles from SunOleic95R into the chromosome of ICGV06100, a high oil content peanut breeding line. In the marker-assisted backcrossing-introgression lines, a 97% increase in oleic acid, and a 92% reduction in linoleic acid content was observed in comparison to the recurrent parent. Besides, the oleic/linoleic ratio was increased to 25 with respect to the recurrent parent, which was only 1.2. The most significant outcome was the stable expression of oil-content, oleic acid, linoleic acid, and palmitic acid in the marker-assisted backcrossing-introgression lines over the locations. No significant difference was observed between high oleic and normal oleic in peanuts for seedling traits except germination percentage. In addition, marker-assisted backcrossing-introgression lines exhibited higher yield and resistance to foliar fungal diseases, i.e., late leaf spot and rust.

    Item Type: Article
    Divisions: Research Program : Asia
    Research Program : Genetic Gains
    CRP: UNSPECIFIED
    Uncontrolled Keywords: Groundnut, Peanut, Legume, Oleic Acid
    Subjects: Mandate crops > Groundnut
    Others > Food Legumes
    Others > Oilseeds
    Others > Genetics and Genomics
    Others > Food and Nutrition
    Depositing User: Mr Arun S
    Date Deposited: 13 Feb 2020 06:22
    Last Modified: 13 Feb 2020 06:22
    URI: http://oar.icrisat.org/id/eprint/11370
    Official URL: https://doi.org/10.1371/journal.pone.0226252
    Projects: UNSPECIFIED
    Funders: Integrated Scheme of Oilseeds, Pulses, Oilpalm and Maize (ISOPOM), Department of Agriculture and Co-operation (DAC), Ministry of Agriculture, Government of India.
    Acknowledgement: Authors acknowledge the financial support received from Integrated Scheme of Oilseeds, Pulses, Oilpalm and Maize (ISOPOM), Ministry of Agriculture and Farmers Welfare, the Government of India.
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