Next‐generation sequencing identified genomic region and diagnostic markers for resistance to bacterial wilt on chromosome B02 in peanut ( Arachis hypogaea L.)

    Luo, H and Pandey, M K and Khan, A W and Wu, B and Guo, J and Ren, X and Zhou, X and Chen, Y and Chen, W and Huang, L and Liu, N and Lei, Y and Liao, B and Varshney, R K and Jiang, H (2019) Next‐generation sequencing identified genomic region and diagnostic markers for resistance to bacterial wilt on chromosome B02 in peanut ( Arachis hypogaea L.). Plant Biotechnology Journal (TSI), 17 (12). pp. 2356-2369. ISSN 1467-7644

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    Abstract

    Bacterial wilt, caused by Ralstonia solanacearum, is a devastating disease affecting over 350plant species. A few peanut cultivars were found to possess stable and durable bacterial wiltresistance (BWR). Genomics-assisted breeding can accelerate the process of developing resistant cultivars by using diagnostic markers. Here, we deployed sequencing-based trait mappingapproach, QTL-seq, to discover genomic regions, candidate genes and diagnostic markers for BWR in a recombination inbred line population (195 progenies) of peanut. The QTL-seq analysis identified one candidate genomic region on chromosome B02 significantly associated with BWR.Mapping of newly developed single nucleotide polymorphism (SNP) markers narrowed down theregion to 2.07 Mb and confirmed its major effects and stable expressions across three environments. This candidate genomic region had 49 non synonymous SNPs affecting 19 putative candidate genes including seven putative resistance genes (R-genes). Two diagnostic markers were successfully validated in diverse breeding lines and cultivars and could be deployed in genomics-assisted breeding of varieties with enhanced BWR.

    Item Type: Article
    Divisions: Research Program : Genetic Gains
    CRP: CGIAR Research Program on Grain Legumes and Dryland Cereals (GLDC)
    Uncontrolled Keywords: Peanut, Bacterial wilt resistance, QTL-seq, Candidate genes, Diagnostic markers
    Subjects: Others > Plant Breeding
    Mandate crops > Groundnut
    Others > Genetics and Genomics
    Others > Plant Disease
    Others > Legume Crops
    Depositing User: Mr Arun S
    Date Deposited: 20 Nov 2019 05:46
    Last Modified: 20 Nov 2019 05:58
    URI: http://oar.icrisat.org/id/eprint/11336
    Official URL: https://doi.org/10.1111/pbi.13153
    Projects: UNSPECIFIED
    Funders: Youth Chenguang Project of Science and Technology of Wuhan City, National Natural Science Foundations of China
    Acknowledgement: This study was supported by the Youth Chenguang Project of Science and Technology of Wuhan City (grant number 2017050304010285) and the National Natural Science Foundations of China (grant numbers 31601340, 31571713, 31761143005 and 31461143022). This work has been undertaken as part of the CGIAR Research Program on Grain Legumes and Dryland Cereals (GLDC). ICRISAT is a member of CGIAR Consortium. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. We thank Prof. Xinyou Zhang from Henan Academy of Agricultural Sciences, China for providing the peanut cultivar Yuanza 5.
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