Mapping Quantitative Trait Loci for Carotenoid Concentration in Three F2 Populations of Chickpea

Rezaei, M K and Deokar, A A and Arganosa, G and Roorkiwal, M and Pandey, S K and Warkentin, T D and Varshney, R K and Tar′an, B (2019) Mapping Quantitative Trait Loci for Carotenoid Concentration in Three F2 Populations of Chickpea. The Plant Genome (TSI), 12 (3). pp. 1-12. ISSN 1940-3372

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Abstract

Three F2 populations derived from crosses between cultivars with green and yellow cotyledon colors were used to identify quantitative trait loci (QTLs) associated with carotenoid components in chickpea (Cicer arietinum L.) seeds developed by the Crop Development Centre (CDC). Carotenoids including violaxanthin, lutein, zeaxanthin, b-cryptoxanthin, and b-carotene were assessed in the F2:3 seeds via high-performance liquid chromatography (HPLC). In the ‘CDC Jade’ × ‘CDC Frontier’ population, 1068 bin markers derived from the 50K Axiom CicerSNP array were mapped onto eight linkage groups (LGs). Eight QTLs, including two each for b-carotene and zeaxanthin and one each for total carotenoids, b-cryptoxanthin, b-carotene, and violaxanthin were identified in this population. In the ‘CDC Cory’ × ‘CDC Jade’ population, 694 bin markers were mapped onto eight LGs and one partial LG. Quantitative trait loci for b-cryptoxanthin, b-carotene, violaxanthin, lutein, and total carotenoids were identified on LG8. A map with eight LGs was developed from 581 bin markers in the third population derived from the ‘ICC4475’ × ‘CDC Jade’ cross. One QTL for b-carotene and four QTLs, one each for b-cryptoxanthin, b-carotene, lutein, and total carotenoids, were identified in this population. The highest phenotypic variation explained by the QTLs was for b-carotene, which ranged from 58 to 70% in all three populations. A major gene for cotyledon color was mapped on LG8 in each population. A significant positive correlation between cotyledon color and carotenoid concentration was observed. Potential candidate genes associated with carotenoid components were obtained and their locations on the chickpea genome are presented.

Item Type:	Article
Divisions:	Research Program : Genetic Gains
CRP:	UNSPECIFIED
Uncontrolled Keywords:	Quantitative trait locus, QTLs, Chickpea seeds, Genomics
Subjects:	Others > Plant Genetics Others > Plant Breeding Mandate crops > Chickpea Others > Genetics and Genomics
Depositing User:	Mr Arun S
Date Deposited:	24 Sep 2019 09:19
Last Modified:	24 Sep 2019 10:18
URI:	http://oar.icrisat.org/id/eprint/11315
Official URL:	https://doi.org/10.3835/plantgenome2019.07.0067
Projects:	UNSPECIFIED
Funders:	Saskatchewan Pulse Growers, The Saskatchewan Ministry of Agriculture, Saskatchewan Innovation and Opportunity Scholarship
Acknowledgement:	This study was funded by the Saskatchewan Pulse Growers, the Saskatchewan Ministry of Agriculture, and the Saskatchewan Innovation and Opportunity Scholarship. We thank Parvaneh Hashemi, Carmen Breitkreutz, and Barry Goetz for their technical assistance. We also appreciate support from the Pulse Breeding staff at the University of Saskatchewan.
Links:	Google Scholar

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