Molecular dissection of genetic diversity in pigeonpea [Cajanus cajan (L) Millsp.] minicore collection

Muniswamy, S and Lokesha, R and Saxena, R K and Fakrudin, B and Patel, K and Yamanura, - (2019) Molecular dissection of genetic diversity in pigeonpea [Cajanus cajan (L) Millsp.] minicore collection. Legume Research - An International Journal (TSI), 42 (1). pp. 32-38. ISSN 0250-5371

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Abstract

The present investigation was carried out using 191genotypes as mini core collections of pigeonpea along with 5 check varieties to know the genetic diversity at molecular level. Significant variation was observed by the way of analysis of variance for nine characters viz., days to 50% flowering, days to maturity, plant height, number of branches per plant, pod bearing length, number of pods per plant, number of seeds per pod, seed yield per plant and hundred seed weight. Molecular diversity using 18 polymorphic simple sequence repeat (SSR) markers divided genotypes into 15 clusters, of which ICP11059 and AK-101 were solitary, indicating their distinctiveness among all genotypes. Similarly, BSMR-533, JKM-7, RVK-285, ICP-1126, ICP-348, ICP-6859 and ICP-7869 were found distinct among the genotypes. Geographical origin based diversity separated Indian and non Indian genotypes. The Un weighted Pair Group Method with Arithmetic mean (UPGMA) based dendrogram indicated distinctiveness of ICP-13633 and Bennur local, as they formed solitary cluster. The SSR marker CcM 602, as it could differentiate 4 genotypes at different base pair size can be used for identification and finger printing of genotypes.

Item Type:	Article
Divisions:	Research Program : Genetic Gains
CRP:	UNSPECIFIED
Uncontrolled Keywords:	Cajanus cajan, Genetic diversity, Minicore, Molecular characterization, Pigeonpea
Subjects:	Mandate crops > Pigeonpea Others > Genetics and Genomics
Depositing User:	Mr Ramesh K
Date Deposited:	06 Sep 2019 09:04
Last Modified:	06 Sep 2019 09:04
URI:	http://oar.icrisat.org/id/eprint/11291
Official URL:	https://doi.org/10.18805/LR-3817
Projects:	UNSPECIFIED
Funders:	UNSPECIFIED
Acknowledgement:	The laboratory work on molecular diversity was carried out at Dept.of Biotechnology, UAS, Dharwad and Centre of Excellence in Genomics at ICRISAT, Patancheru, Hyderabad. (Under the guidance of Dr.Rajeev K. Varshney). The seed material was obtained from NICRA net work project.
Links:	Google Scholar

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