Utilization of Molecular Marker Based Genetic Diversity Patterns in Hybrid Parents to Develop Better Forage Quality Multi-Cut Hybrids in Pearl Millet

Ponnaiah, G and Gupta, S K and Blummel, M and Marappa, M and Pichaikannu, S and Das, R R and Rathore, A (2019) Utilization of Molecular Marker Based Genetic Diversity Patterns in Hybrid Parents to Develop Better Forage Quality Multi-Cut Hybrids in Pearl Millet. Agriculture, 9(5) (97). pp. 1-21. ISSN 2077-0472

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Abstract

Genetic diversity of 130 forage-type hybrid parents of pearl millet was investigated based on multiple season data of morphological traits and two type of markers: SSRs (Simple sequence repeats) and GBS identified SNPs (Genotyping by sequencing-Single nucleotide polymorphism). Most of the seed and pollinator parents clustered into two clear-cut separate groups based on marker based genetic distance. Significant variations were found for forage related morphological traits at different cutting intervals (first and second cut) in hybrid parents. Across two cuts, crude protein (CP) varied from 11% to 15%, while in vitro organic matter digestibility (IVOMD) varied from 51% to 56%. Eighty hybrids evaluated in multi-location trial along with their parents for forage traits showed that significant heterosis can be realized for forage traits. A low but positive significant correlation found between SSR based genetic distance (GD between parents of hybrid) and heterosis for most of the forage traits indicated that SSR-based GD can be used for predicting heterosis for GFY, DFY and CP in pearl millet. An attempt was made to associate marker-based clusters with forage quality traits, to enable breeders select parents for crossing purposes in forage breeding programs.

Item Type:	Article
Divisions:	Research Program : Asia
CRP:	CGIAR Research Program on Dryland Cereals
Uncontrolled Keywords:	Genetic diversity; markers; forage yield; crude protein; in vitro organic matter digestibility; pearl millet
Subjects:	Others > Plant Breeding Mandate crops > Millets > Pearl Millet Others > Genetics and Genomics
Depositing User:	Mr Ramesh K
Date Deposited:	25 Jun 2019 10:33
Last Modified:	25 Jun 2019 10:33
URI:	http://oar.icrisat.org/id/eprint/11132
Official URL:	https://doi.org/10.3390/agriculture9050097
Projects:	UNSPECIFIED
Funders:	UNSPECIFIED
Acknowledgement:	This research work was carried out as part of a Ph.D. thesis of Govintharaj, submitted to Tamil Nadu Agricultural University, Coimbatore, Tamil Nadu. It was financially supported by the ICRISAT-Pearl Millet Hybrid Parents Research Consortium (PMHPRC) and conducted under CGIAR, Research Program on Dryland Cereals. We also acknowledge Mahender Thudi from the ICRISAT-CEG for his assistance in lab work. We also thank K.V.S. Prasad and Ramakrishna Reddy from ICRISAT-ILRI for sample preparation and NIRS analysis.
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