Ha, J and Shim, S and Lee, T and Kang, Y J and Hwang, W J and Jeong, H and Laosatit, K and Lee, J and Kim, S K and Satyawan, D and Lestari, P and Yoon, M Y and Kim, M Y and Chitikineni, A and Tanya, P and Somta, P and Srinives, P and Varshney, R K and Lee, S H (2019) Genome sequence of Jatropha curcas L., a non-edible biodiesel plant, provides a resource to improve seed-related traits. Plant Biotechnology Journal (TSI), 17 (2). pp. 517-530. ISSN 14677644
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Abstract
Jatropha curcas (physic nut), a non‐edible oilseed crop, represents one of the most promising alternative energy sources due to its high seed oil content, rapid growth and adaptability to various environments. We report ~339 Mbp draft whole genome sequence of J. curcas var. Chai Nat using both the PacBio and Illumina sequencing platforms. We identified and categorized differentially expressed genes related to biosynthesis of lipid and toxic compound among four stages of seed development. Triacylglycerol (TAG), the major component of seed storage oil, is mainly synthesized by phospholipid:diacylglycerol acyltransferase in Jatropha, and continuous high expression of homologs of oleosin over seed development contributes to accumulation of high level of oil in kernels by preventing the breakdown of TAG. A physical cluster of genes for diterpenoid biosynthetic enzymes, including casbene synthases highly responsible for a toxic compound, phorbol ester, in seed cake, was syntenically highly conserved between Jatropha and castor bean. Transcriptomic analysis of female and male flowers revealed the up‐regulation of a dozen family of TFs in female flower. Additionally, we constructed a robust species tree enabling estimation of divergence times among nine Jatropha species and five commercial crops in Malpighiales order. Our results will help researchers and breeders increase energy efficiency of this important oil seed crop by improving yield and oil content, and eliminating toxic compound in seed cake for animal feed.
| Item Type: | Article |
|---|---|
| Divisions: | Research Program : Genetic Gains |
| CRP: | UNSPECIFIED |
| Uncontrolled Keywords: | oil synthesis, phorbol ester, biodiesel, seed cake, energy production, phylogenetic analysis |
| Subjects: | Others > Genetics and Genomics Others > Jatropha |
| Depositing User: | Mr Ramesh K |
| Date Deposited: | 14 Feb 2019 09:13 |
| Last Modified: | 17 Jan 2020 03:07 |
| URI: | http://oar.icrisat.org/id/eprint/11063 |
| Official URL: | http://dx.doi.org/10.1111/pbi.12995 |
| Projects: | UNSPECIFIED |
| Funders: | UNSPECIFIED |
| Acknowledgement: | This research was supported by a grant from the Next Generation BioGreen 21 Program (Code No. PJ01326101), Rural Development Administration, Republic of Korea and Science and Technology Support Program through the National Research Foundation of Korea(NRF) funded by the Ministry of Science and ICT(MSIT) (2013K1A3A9A01044312). Hwang, Won Joo is affiliated with CJ Food R&D, Suwon 16495, Republic of Korea. The authors declare no conflict of interest. |
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