Streptomyces sp. as plant growth-promoters and host-plant resistance inducers against Botrytis cinerea in chickpea

Vijayabharathi, R and Gopalakrishnan, S and Sathya, A and Vasanth Kumar, M and Srinivas, V and Mamta, S (2018) Streptomyces sp. as plant growth-promoters and host-plant resistance inducers against Botrytis cinerea in chickpea. Biocontrol Science and Technology (TSI). pp. 1-24. ISSN 0958-3157

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Abstract

Two hundred and fifty seven actinobacteria, isolated from five different rhizosphere soils of chickpea, were evaluated for their antagonistic potential against Botrytis cinerea, causal agent of Botrytis grey mold (BGM) disease in chickpea, by dual culture assay. Of them, three most promising isolates (ATIRS43, ATIRS65 and ARRS10) with highest inhibitory activity (67–77%) were identified as Streptomyces sp. These selected isolates induced growth of chickpea genotype JG11 as a consortium rather than an individual inoculum. Co-inoculation of the selected Streptomyces sp. with Mesorhizobium ciceri UPM-Ca7T enhanced nodulation and nitrogenase activity in five chickpea genotypes (ICCV2, ICCV10, ICC4958, Annigeri and JG11). The selected Streptomyces sp. significantly reduced the disease incidence caused by B. cinerea by 28–47% over the un-inoculated control across the chickpea genotypes ICC4954 (susceptible), ICCV05530 (moderately resistant) and JG11 (unknown resistance). The Streptomyces sp. were also able to induce host-plant resistance, irrespective of the genotype, through the induction of various antioxidant enzymes and phenolics. Phenolic profiling of B. cinerea-affected and Streptomyces treated plants of ICCV05530 further confirmed host-plant resistance traits. This study indicated that the selected Streptomyces sp. have the potential for biological control of BGM disease in chickpea.

Item Type:	Article
Divisions:	Research Program : Asia
CRP:	CGIAR Research Program on Grain Legumes and Dryland Cereals (GLDC)
Uncontrolled Keywords:	Botrytis cinerea, Streptomyces, chickpea, plant growth-promotion, host-plant resistance, antioxidants
Subjects:	Others > Crop Improvement Mandate crops > Chickpea Others > Plant Growth
Depositing User:	Mr Ramesh K
Date Deposited:	28 Aug 2018 08:40
Last Modified:	28 Aug 2018 08:40
URI:	http://oar.icrisat.org/id/eprint/10846
Official URL:	http://dx.doi.org/10.1080/09583157.2018.1515890
Projects:	UNSPECIFIED
Funders:	Young Scientist scheme of Science and Engineering Research Board (SERB), DST, India
Acknowledgement:	This work was undertaken as part of the CGIAR Research Program on Grain Legumes and Dryland Cereals. ICRISAT is a member of CGIAR Consortium. We thank Dr K Sivasakthi, Crop Physiology, ICRISAT, for statistical assistance, Dr G Senthilraja Centre of Excellence in Millets, Tamil Nadu Agricultural University, Tiruvannamalai for AUDPC calculation. Technical assistance of Mr Rameshwar for BGM experiments and Mr S Suraj Prasad for HPLC analysis is acknowledged. We also thank our lab members M/s PVS Prasad and A Jabbar for technical assistance. Funding This study is supported by Young Scientist scheme of Science and Engineering Research Board (SERB), DST, India through [grant number SB/YS/LS-345/2013] and author RV is grateful to this.
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