Deep sequencing of small RNAs reveals ribosomal origin of microRNAs in Oryza sativa and their regulatory role in high temperature

Mangrauthia, S K and Sailaja, B and Pusuluri, M and Jena, B and Prasanth, V V and Agarwal, S and Senguttuvel, P and Sarla, N and Ravindra Babu, V and Subrahmanyam, D and Voleti, S R (2018) Deep sequencing of small RNAs reveals ribosomal origin of microRNAs in Oryza sativa and their regulatory role in high temperature. Gene Reports (TSI), 11. pp. 270-278. ISSN 24520144

PDF - Published Version Restricted to ICRISAT users only Download (2MB)

Abstract

MicroRNAs are small noncoding regulatory RNAs which control gene expression by mRNA degradation or translational repression. They are significant molecular players regulating important biological processes such as developmental timing and stress response. We report here the discovery of miRNAs derived from ribosomal DNA using the small RNA datasets of 16 deep sequencing libraries of rice. Twelve putative miRNAs were identified based on highly stringent criteria of novel miRNA prediction. Surprisingly, 10 putative miRNAs (mi_7403, mi_8435, mi_12675, mi_4266, mi_4758, mi_4218, mi_8200, mi_4644, mi_14291, mi_16235) originated from rDNA of rice chromosome 9. Expression analysis of putative miRNAs and their target genes in heat tolerant and susceptible rice cultivars in control and high temperature treated seedlings revealed differential regulation of rDNA derived miRNAs. This is the first report of rDNA derived miRNAs in rice which indicates their role in gene regulation during high temperature stress in plants. Further studies in this area will open new research challenges and opportunities to broaden our knowledge on gene regulation mechanisms.

Item Type:	Article
Divisions:	UNSPECIFIED
CRP:	UNSPECIFIED
Uncontrolled Keywords:	Rice, Novel microRNAs, Ribosomal DNA, Rice chromosome 9, Gene expression, High temperature stress, RNA sequencing, heat stress, gene regulation mechanisms, rDNA-miRNAs
Subjects:	Others > Abiotic Stress Others > Rice Others > Genetics and Genomics Others > Plant Physiology
Depositing User:	Mr Ramesh K
Date Deposited:	22 Jun 2018 09:38
Last Modified:	06 Feb 2019 09:48
URI:	http://oar.icrisat.org/id/eprint/10758
Official URL:	http://dx.doi.org/10.1016/j.genrep.2018.05.002
Projects:	UNSPECIFIED
Funders:	UNSPECIFIED
Acknowledgement:	Authors are highly thankful to the Director, IIRR, for his kind support. Financial support received from ICAR-NICRA (National Innovations on Climate and Resilient Agriculture) project is acknowledged. Authors are also thankful to Nucleome Informatics Private Limited, Hyderabad for their Bioinformatics support.
Links:	Google Scholar

View Statistics

Actions (login required)

View Item