Saxena, R K and Patel, K and Sameer Kumar, C V and Tyagi, K and Saxena, K B and Varshney, R K (2018) Molecular mapping and inheritance of restoration of fertility (Rf) in A4 hybrid system in pigeonpea (Cajanus cajan (L.) Millsp.). Theoretical and Applied Genetics (TSI). pp. 1-10. ISSN 0040-5752
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Abstract
Key message We report molecular mapping and inheritance of restoration of fertility (Rf) in A4 hybrid system in pigeonpea. We have also developed PCR-based markers amenable to low-cost genotyping to identify fertility restorer lines. Abstract Commercial hybrids in pigeonpea are based on A4 cytoplasmic male sterility (CMS) system, and their fertility restoration is one of the key prerequisites for breeding. In this context, an effort has been made to understand the genetics and identify quantitative trait loci (QTL) associated with restoration of fertility (Rf). One F2 population was developed by crossing CMS line (ICPA 2039) with fertility restorer line (ICPL 87119). Genetic analysis has shown involvement of two dominant genes in regulation of restoration of fertility. In parallel, the genotyping-by-sequencing (GBS) approach has generated ~ 33 Gb data on the F2 population. GBS data have provided 2457 single nucleotide polymorphism (SNPs) segregating across the mapping population. Based on these genotyping data, a genetic map has been developed with 306 SNPs covering a total length 981.9 cM. Further QTL analysis has provided the region flanked by S8_7664779 and S8_6474381 on CcLG08 harboured major QTL explained up to 28.5% phenotypic variation. Subsequently, sequence information within the major QTLs was compared between the maintainer and the restorer lines. From this sequence information, we have developed two PCR-based markers for identification of restorer lines from non-restorer lines and validated them on parental lines of hybrids as well as on another F2 mapping population. The results obtained in this study are expected to enhance the efficiency of selection for the identification of restorer lines in hybrid breeding and may reduce traditional time-consuming phenotyping activities.
| Item Type: | Article |
|---|---|
| Divisions: | Research Program : Genetic Gains Research Program : Asia |
| CRP: | CGIAR Research Program on Grain Legumes and Dryland Cereals (GLDC) |
| Uncontrolled Keywords: | Molecular mapping, quantitative trait loci, hybrid system, pigeonpea, hybrid breeding, Mapping population, Genetic mapping, SNPs, QTL analysis, QTL Mapping |
| Subjects: | Others > Molecular Biology Others > Plant Breeding Mandate crops > Pigeonpea |
| Depositing User: | Mr Ramesh K |
| Date Deposited: | 15 May 2018 04:49 |
| Last Modified: | 09 Apr 2019 03:45 |
| URI: | http://oar.icrisat.org/id/eprint/10661 |
| Official URL: | http://dx.doi.org/10.1007/s00122-018-3101-y |
| Projects: | UNSPECIFIED |
| Funders: | UNSPECIFIED |
| Acknowledgement: | Authors are thankful to the Biotechnology Industry Partnership Programme (BIPP), Department of Biotechnology, Government of India and Bill & Melinda Gates Foundation for financial support. Authors would also like to thank Vinay Kumar, Vanika Gerg, Jimmy Obala, Aamir Khan and Sandip Kale for their help in the present study. This work has been undertaken as part of the CGIAR Research Program on Grain Legumes and Dryland Cereals. ICRISAT is a member of CGIAR Consortium. |
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