Genetic polymorphisms among and between blast disease resistant and susceptible finger millet, Eleusine coracana (L.) Gaertn.

Saha, D and Rana, R S and Arya, L and Verma, M and Gowda, M V C and Upadhyaya, H D (2017) Genetic polymorphisms among and between blast disease resistant and susceptible finger millet, Eleusine coracana (L.) Gaertn. Plant Genetic Resources, 15 (04). pp. 355-365. ISSN 1479-2621

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Abstract

Fungal blast disease is one of the major constraints in finger millet production. Breeding for disease resistance in finger millet, needs characterization of genetic polymorphism among and between the resistant and susceptible genotypes. In total, 67 finger millet genotypes, which are resistant or susceptible to fungal blast disease, were analysed using sequence-related amplified polymorphism (SRAP) and simple sequence repeat (SSR) markers to assess genetic variations and select diverse parents. Twelve each of SRAP and SSR primers produced 95.1 and 93.1% polymorphic bands and grouped them into unweighted pair-group method with arithmetic average clusters. Two of the finger millet genotypes, IE 4709 (blast resistant) and INDAF 7 (susceptible) were distinguished as most diverse genotypes as parents. Several genotype-specific bands observed with SSR primers are potential in developing genotype-specific markers. A high genetic diversity within the resistant and susceptible genotypes, rather than between them, was revealed through Nei's gene diversity (h) index and analysis of molecular variance. The finding helps us to understand the extent of genetic polymorphism between blast disease resistant and susceptible finger millet genotypes to exploit in resistance breeding programs.

Item Type:	Article
Divisions:	Research Program : Genetic Gains
CRP:	UNSPECIFIED
Uncontrolled Keywords:	Genetic polymorphisms, Finger millet, Fungal blast disease, Genetic variability, Resistant and susceptible genotypes, Sequence related amplified polymorphism (SRAP), Simple sequence repeat (SSR)
Subjects:	Others > Plant Genetics Mandate crops > Millets Mandate crops > Millets > Finger Millet Others > Plant Disease
Depositing User:	Mr Ramesh K
Date Deposited:	31 Oct 2017 06:12
Last Modified:	31 Oct 2017 06:13
URI:	http://oar.icrisat.org/id/eprint/10240
Official URL:	http://dx.doi.org/10.1017/S1479262116000010
Projects:	UNSPECIFIED
Funders:	UNSPECIFIED
Acknowledgement:	Authors thank National Bureau of Plant Genetic Resources, New Delhi, the All India Co-ordinated Small Millets Improvement Project, Bengaluru and the International Crops Research Institute for the Semi-Arid Tropics, Hyderabad for providing finger millet accessions. The research support provided by the Head, Division of Genomic Resources and Director, NBPGR is also acknowledged. Authors are grateful to Dr Savithri Nambeesan, Assistant Research Scientist, University of Georgia, Athens, USA for language correction of the article.
Links:	Google Scholar

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