QTL mapping of pearl millet rust resistance using an integrated DArT- and SSR-based linkage map

Ambawat, S and Senthilvel, S and Hash, C T and Nepolean, T and Rajaram, V and Eshwar, K and Sharma, R and Thakur, R P and Rao, V P and Yadav, R C and Srivastava, R K (2016) QTL mapping of pearl millet rust resistance using an integrated DArT- and SSR-based linkage map. Euphytica, 209 (2). pp. 461-476. ISSN 1573-5060

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Rust, caused by the fungus Puccinia substriata var. indica, is one of the most important production constraints of pearl millet worldwide, leading to grain yield losses of up to 76 % as well as major losses in fodder yield and quality. Here, we report the development of a linkage map integrating Diversity Arrays Technology (DArT) markers and simple sequence repeat (SSR) markers, using this to identify quantitative trait loci (QTLs) for pearl millet rust resistance. Genotyping data from 256 DArT and 70 SSR markers on 168 F7 recombinant inbred lines from cross 81B-P6 × ICMP 451-P8 were used to construct a linkage map comprised of 286 loci (229 DArT and 57 SSR markers) spanning a total length of 740.3 cM (Haldane) with an average adjacent marker distance of 2.7 cM. Linkage group 7 (LG7) (153.5 cM) was the longest and LG6 the shortest (45.0 cM). The map was used to identify a major QTL for rust resistance with an LOD score of 27 on LG1, which explained 58 % of the observed phenotypic variation. In addition, two putative modifiers of small effect were detected, one each on LG4 and LG7. The novel rust resistance QTL identified on LG1 is thought to confer a durable slow-rusting phenotype, which is still effective in India more than 20 years after it was first deployed in the previously popular single-cross hybrid MH 179 (ICMH 451). The flanking markers reported here provide a framework for marker-assisted selection and possible future map-based cloning of this resistance gene.

Item Type: Article
Divisions: RP-Dryland Cereals
CRP: CGIAR Research Program on Dryland Cereals
Uncontrolled Keywords: Pennisetum glaucum, Molecular markers, Diversity Arrays Technology, Linkage map, Rust resistance, QTL mapping, Pearl millet
Subjects: Mandate crops > Millets > Pearl Millet
Depositing User: Mr Ramesh K
Date Deposited: 01 Apr 2016 08:42
Last Modified: 11 Apr 2017 05:12
URI: http://oar.icrisat.org/id/eprint/9400
Official URL: http://dx.doi.org/10.1007/s10681-016-1671-9
Acknowledgement: This study was supported by the Generation Challenge Programme (GCP) of Consultative Group on International Agricultural Research (CGIAR) and Department of Biotechnology (DBT), Government of India. We thank DArT P/L, Australia, for providing the technical knowhow, software and helpful discussion. The authors also acknowledge the help provided by the pathology and pearl millet breeding staff at ICRISAT-Patancheru during greenhouse screening. This work was published as part of the CGIAR Research ProgramonDrylandCereals. ICRISATisamemberof the CGIAR Consortium.
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