Evaluation of genetic diversity in Magnaporthe grisea populations adapted to finger millet using simple sequence repeats (SSRs) markers

Kiran Babu, T and Sharma, R and Upadhyaya, H D and Reddy, P N and Deshpande, S P and Senthilvel, S and Sarma, N D R K and Thakur, R P (2013) Evaluation of genetic diversity in Magnaporthe grisea populations adapted to finger millet using simple sequence repeats (SSRs) markers. Physiological and Molecular Plant Pathology, 84. pp. 10-18. ISSN 0885-5765

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Abstract

Finger millet blast caused by Magnaporthe grisea (anamorph: Pyricularia grisea) is a great threat to finger millet production worldwide. Genetic diversity and population structure of 72 M. grisea isolates collected from finger millet (56), foxtail millet (6), pearl millet (7) and rice (3) from major crop growing areas in India was studied using 24 SSR markers. None of the SSRs detected polymorphism in the M. grisea isolates from pearl millet. Seventeen SSR markers were polymorphic in the 65 non pearl millet isolates and detected 105 alleles, of which one was rare, 83 common, 9 frequent and 12 most frequent. A model-based population structure analysis of the genomic data identified two distinct populations with varying levels of ancestral admixtures among the 65 M. grisea isolates. Analysis of molecular variance (AMOVA) indicated that 52% of the total variation among the isolates used in this study was due to differences between the pathogen populations adapted to different hosts, 42% was due to differences in the isolates from the same host, and the remaining 6% due to heterozygosity within isolates. High genetic variability present in M. grisea isolates calls for the continuous monitoring of M. grisea populations anticipating blast resistance breakdown in finger millet cultivars grown in India.

Item Type: Article
Divisions: UNSPECIFIED
CRP: UNSPECIFIED
Uncontrolled Keywords: Genetic diversity; Simple sequence repeats; Magnaporthe grisea; Eleusine coracana
Subjects: Mandate crops > Millets
Depositing User: Mr Sanat Kumar Behera
Date Deposited: 06 Jul 2013 06:30
Last Modified: 17 Mar 2014 06:15
URI: http://oar.icrisat.org/id/eprint/6920
Official URL: http://dx.doi.org/10.1016/j.pmpp.2013.06.001
Projects: Sustainable conservation andutilization of genetic resources of two underutilized crops-finger millet and foxtail millet-to enhance productivity, nutrition and income in Africa and Asia
Funders: Federal Ministry for Economic Cooperation and Development, Germany
Acknowledgement: The authors gratefully acknowledge the financial support from the BMZ/GTZ project on “Sustainable conservation and utilization of genetic resources of two underutilized crops-finger millet and foxtail millet-to enhance productivity, nutrition and income in Africa and Asia” funded by the Federal Ministry for Economic Cooperation and Development (BMZ), Germany to carry out this work.
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