Serodiagnosis of Peanut Bud Necrosis Virus of Groundnut Occurring in North-Easternkarnataka

Srinivasaraghavan, - and Sunkad, A G and Mallikarjuna, N and Sudini, H (2012) Serodiagnosis of Peanut Bud Necrosis Virus of Groundnut Occurring in North-Easternkarnataka. Bioinfolet, 9 (2). pp. 91-95. ISSN 0976-4755

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The groundnut samples with peanut bud necrosis were collected from different parts of north eastern Karnataka and subjected to DAC-ELISA technique using polyclonal antiserum of PBNV and TSWV. All samples reacted positively with PBNV, while gave negative reaction against TSWV antisera. The weed samples prevailing in groundnut ecosystem, subjected to DAC-ELISA against PBNV also gave negative reaction. ELISA for different symptomatic PBND groundnut plants revealed that the primary symptomatic plants contained higher virus concentration. Furthermore, PBNV infected leaves with primary symptoms had highest virus titre. The study revealed that the virus causing bud necrosis in groundnut and tomato in north eastern Karnataka was PBNV. There was no natural infection of PBNV in major weeds prevailing in groundnut fields.

Item Type: Article
Subjects: Mandate crops > Groundnut
Depositing User: Mr Sanat Kumar Behera
Date Deposited: 18 Jun 2012 05:27
Last Modified: 18 Jun 2012 05:27
Official URL:
Acknowledgement: We are thankful to International crop research institute for semi arid tropics ( ICRISAT ), Hyderabad for providing facilities for carrying experiment related to serology.
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