New eSSR and gSSR markers added to Australian barley maps

Willsmore, K L and Eckermann, P and Varshney, R K and Graner, A and Langridge, P and Pallotta, M and Cheong, J and Williams, K J (2006) New eSSR and gSSR markers added to Australian barley maps. Australian Journal of Agricultural Research, 57 (9). pp. 953-959. ISSN 0004-9409

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To enhance genetic maps of barley previously developed in Australia for identifying markers useable in molecular breeding, a new set of simple sequence repeat (SSR) and indel markers was added to the maps. These markers were developed through (i) database mining of barley expressed sequence tag (EST) sequences, (ii) comparative barley-rice genome analysis, and (iii) screening of a genomic library with SSR probes. The primer set selected for this study comprised 216 EST-SSR (eSSR) and 25 genomic SSR (gSSR) markers, which were screened for polymorphism on 4 doubled haploid (DH) or recombinant inbred line (RIL) populations. In total, 81 new markers were added to the maps, with good coverage on all 7 chromosomes, except 6H, which only had 2 newmarkers added. The marker order of previously published mapswas re-evaluated by comparing recombination fractions calculated by 2 methods to discover the best position for each marker. The new SSR markers were then added to the updated maps. Several of these new markers are linked to important barley disease resistance genes such as those for cereal cyst nematode, spot form of net blotch, and leaf scald resistance, and are readily useable for marker-assisted barley breeding.

Item Type: Article
Uncontrolled Keywords: microsatellite, Hordeum vulgare, genetic mapping, marker-assisted selection
Subjects: Others > Genetics and Genomics
Others > Agriculture-Farming, Production, Technology, Economics
Depositing User: Library ICRISAT
Date Deposited: 21 Oct 2011 05:59
Last Modified: 21 Oct 2011 06:00
Official URL:
Acknowledgement: UNSPECIFIED

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