New eSSR and gSSR markers added to Australian barley maps

    Willsmore, K L and Eckermann, P and Varshney, R K and Graner, A and Langridge, P and Pallotta, M and Cheong, J and Williams, K J (2006) New eSSR and gSSR markers added to Australian barley maps. Australian Journal of Agricultural Research, 57 (9). pp. 953-959. ISSN 0004-9409

    [img] PDF - Published Version
    Restricted to ICRISAT users only
    Download (144kB) | Request a copy

    Abstract

    To enhance genetic maps of barley previously developed in Australia for identifying markers useable in molecular breeding, a new set of simple sequence repeat (SSR) and indel markers was added to the maps. These markers were developed through (i) database mining of barley expressed sequence tag (EST) sequences, (ii) comparative barley-rice genome analysis, and (iii) screening of a genomic library with SSR probes. The primer set selected for this study comprised 216 EST-SSR (eSSR) and 25 genomic SSR (gSSR) markers, which were screened for polymorphism on 4 doubled haploid (DH) or recombinant inbred line (RIL) populations. In total, 81 new markers were added to the maps, with good coverage on all 7 chromosomes, except 6H, which only had 2 newmarkers added. The marker order of previously published mapswas re-evaluated by comparing recombination fractions calculated by 2 methods to discover the best position for each marker. The new SSR markers were then added to the updated maps. Several of these new markers are linked to important barley disease resistance genes such as those for cereal cyst nematode, spot form of net blotch, and leaf scald resistance, and are readily useable for marker-assisted barley breeding.

    Item Type: Article
    Divisions: UNSPECIFIED
    CRP: UNSPECIFIED
    Uncontrolled Keywords: microsatellite, Hordeum vulgare, genetic mapping, marker-assisted selection
    Subjects: Others > Genetics and Genomics
    Others > Agriculture-Farming, Production, Technology, Economics
    Depositing User: Library ICRISAT
    Date Deposited: 21 Oct 2011 05:59
    Last Modified: 21 Oct 2011 06:00
    URI: http://oar.icrisat.org/id/eprint/3023
    Official URL: http://dx.doi.org/10.1071/AR05384
    Projects: UNSPECIFIED
    Funders: UNSPECIFIED
    Acknowledgement: UNSPECIFIED
    Links:
    View Statistics

    Actions (login required)

    View Item View Item
    OAR@ICRISAT is powered by EPrints 3 which is developed by the School of Electronics and Computer Science at the University of Southampton. More information and software credits.