Random Amplified Microsatellite Polymorphism (RAMPO) Analysis of Chickpea Accessions

Banerjee, H and Leela, A and Seetharama, N (1998) Random Amplified Microsatellite Polymorphism (RAMPO) Analysis of Chickpea Accessions. International Chickpea Newsletter, 5. pp. 16-18. ISSN 0257-2508

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Abstract

DNA was isolated from 4 cultivated Cicer arietinum accessions and one wild C. echinospermum accession using the CTAB method. RAPD analysis with 12 primers revealed 424 amplified DNA bands. The wild species produced a maximum of 34 polymorphic bands, while all cultivars except ICCV92504 produced 2 or 3 polymorphic bands. RAMPO analysis of these accessions using microsatellite probes revealed amplified bands not visible on ethidium [homidium] bromide-stained gel. Combination of RAPD analysis and probing with microsatellite repeats (TG)10, (GATA)4 and (CAC)5 revealed polymorphic and genotype-specific unique DNA banding patterns. Thus, all 5 chickpea accessions could be distinguished using RAMPO.

Item Type: Article
Divisions: UNSPECIFIED
CRP: UNSPECIFIED
Subjects: Mandate crops > Chickpea
Depositing User: Library ICRISAT
Date Deposited: 22 Oct 2011 10:00
Last Modified: 22 Oct 2011 10:00
URI: http://oar.icrisat.org/id/eprint/2947
Official URL:
Projects: UNSPECIFIED
Funders: UNSPECIFIED
Acknowledgement: UNSPECIFIED
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